Bpsbioscience/Fluorogenic HDAC substrate 3/50037/250 nmole
商品编号：
50037
品牌：
bpsbioscience
市场价：
¥4700.00
美元价：
2820.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:
Afluorogenic,acetylatedpeptidesubstrateforclassIHDACs(HDACs1,2,and3)andclass2bHDACs(HDACs6and10).Thissubstratefluorescesatexcitation350-380nm,emissionat440-460nm.Ifautofluorescenceoftheinhibitornearthiswavelengthisanissue,werecommendusingFluorogenicHDACsubstrate2(green),BPScatalog#50038.Forclass2aHDACs(HDACs4,5,7,and9)orHDACs8and11,werecommend#50040.
SuppliedAs:
5mMDMSOsolution(50µl)
Format:
DMSOsolution
Instructionsforuse:
Materials:
HDACAssaybuffer(BPScatalog#50031)
HDACAssaydeveloper(BPScatalog#50030)
HDACSubstrate3(BPScatalog#50037)
HDAC1(0.33µg/µl)(BPScatalog#50051)orotherclass1/2bHDACenzyme
Step1:
AddthefollowingreagentstoalowbindingNUNCblackplate(VWRcatalog#62408-936):
HDACAssayBuffer,38µl
1mg/mlBSA,5µl
200µMHDACsubstrate3,5µl
HDAC1,2µl
Incubateat37°Cfor30min.
Step2:
Stopthereactionbyadding50µlofHDACassaydeveloper(undiluted)andincubatetheplateatroomtemperaturefor15min.
Step3:
Readsamplesinamicrotiter-platereADIngfluorimetercapableofexcitationat350-380nmandemissionat440-460nm.“Blank”value(noenzymenegativecontrol)issubtractedfromallothervalues.
HDACAssaybuffer(BPScatalog#50031)
HDACAssaydeveloper(BPScatalog#50030)
HDACSubstrate3(BPScatalog#50037)
HDAC1(0.33µg/µl)(BPScatalog#50051)orotherclass1/2bHDACenzyme
Step1:
AddthefollowingreagentstoalowbindingNUNCblackplate(VWRcatalog#62408-936):
HDACAssayBuffer,38µl
1mg/mlBSA,5µl
200µMHDACsubstrate3,5µl
HDAC1,2µl
Incubateat37°Cfor30min.
Step2:
Stopthereactionbyadding50µlofHDACassaydeveloper(undiluted)andincubatetheplateatroomtemperaturefor15min.
Step3:
Readsamplesinamicrotiter-platereADIngfluorimetercapableofexcitationat350-380nmandemissionat440-460nm.“Blank”value(noenzymenegativecontrol)issubtractedfromallothervalues.
Storage/StABIlity:
Stableatleast12monthsfromdateofreceipt,whenstoredasdirected
Application(s):
Studyenzymekinetics,andscreensmallmoleculeHDACinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
1.A.Itoetal.(2001)EMBOJ.201331.
2.A.Itoetal.(2002)EMBOJ.216236.
3.N.A.Barlevetal.(2001)Mol.Cell81243.
ApplicationReference(s):
1. Chaperoneco-inducerBGP-15inhibitshistonedeacetylasesandenhancestheheatshockresponsethroughincreasedchromatinaccessibility (2017)
2. Design,synthesis,3Dpharmacophore,QSAR,anddockingstudiesofcarboxylicacidderivativesasHistoneDeacetylaseinhibitorsandcytotoxicagents(2014)
Warning(s):
Protectfromdirectexposuretolight.Avoidfreeze/thawcycles.
ScientificCategory:
Deacetylase
ProductType:
Substrate
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
