Bpsbioscience/Human Interleukin-18/90183-B/25 µg
商品编号：
90183-B
品牌：
bpsbioscience
市场价：
¥4100.00
美元价：
2460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:18kDa
Genbank#:Q14116
a.a:37–193
Background:
IL-18issynthesizedasaBIOLOGicallyinactiveprecursorproteinthathaslimitedbiologicalactivity.TheproformofIL18isprocessedbyoneofthecaspases,Caspase-1,togeneratethe18kDabioactivemolecule.IL-18isproducedduringtheacuteimmuneresponsebymacrophagesandimmaturedendriticcells.Interleukin-18isexpressedbyavarietyofimmuneandnon-immunecells,includingmonocytesandmacrophages.IL-18isoneofthepro-inflammatorycytokines.TheactivitiesofIL-18appeartobespecies-specific.AnimportantfunctionofIL-18istheregulationoffunctionallydistinctsubsetsofT-helpercellsrequiredforcellmediatedimmuneresponses.IL-18functionsasagrowthanddifferentiationfactorforTh1cells.IL-18up-regulatesFASligandmediatedcytotoxicactivitiyofmurinenaturalkillercells.IL-18ispartofacomplexregulatorycircuitinvolvedincausingcelldeathbyapoptosis.
Description:
RecombinantInterleukin-18isadisulfide-linkedmonomerproteinconsistingof158aminoacidresidues,migratesasanapproximately18kDaproteinundernon-reducingandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodinghumanInterleukin-18maturechainwasexpressedinE.coli.
UniProt
Q14116
Synonym(s):
Interleukin18,Interferon-Gamma-InducingFactor,IGIF,IL-18,IL18,IFN
Purity:
≥98%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BiologicalActivity:
TheED50wasdeterminedbythedose-dependentproliferationofhumanPBMCco-stimulatedwithIL-12cellswasfoundtobeintherangeof5ng/ml.
Formulation:
Lyophilizedfrom0.2µmfilteredPBS,pH7.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.TsutsuiH,NakanishiK.Immunotherapy.2012Dec,4(12):1883-94.
2.BarkerBR,etal.CurrOpinImmunol.2011Oct,23(5):591-7
2.BarkerBR,etal.CurrOpinImmunol.2011Oct,23(5):591-7
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
