Bpsbioscience/人白细胞介素-1β/90168-B/10µg
商品编号：
90168-B
品牌：
bpsbioscience
市场价：
¥4100.00
美元价：
2460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:17kDa
Genbank#:P01584
a.a:117–269
Background:
MonocytesarethemainsourceofsecretedIL-1.TheyexpresspredominantlyIL-1betawhilehumankeratinocytesexpresslargeamountsofIL-1alpha.MurinemacrophagesdisplayatransitionfromIL-1betatoIL-1alphaproductionduringmaturationofmonocytesintoinflammatorymacrophages.TherearetwofunctionallyalmostequivalentformsofIL-1,IL-1alphaandIL-1betathatareencodedbytwodifferentgenes.IL1-betaisthepredominantforminhumanswhileitisIL-1alphainmice.BothformsofIL-1bindtothesamereceptorandthereforealsoshowsimilarifnotidenticalBIOLOGicalactivities.TheIL-1betabutnottheIL-1alphaprecursormustbeprocessedbeforeitcanbindtothereceptor.BothformsofIL-1bindtothesamereceptorandthereforealsoshowsimilarifnotidenticalbiologicalactivities.ThereceptorisolatedfromT-cellsisexpressedpredominantlyonT-cellsandcellsofmesenchymalorigin.ItbindsbothtypesofIL-1withequalaffinity.ThistypeiscalledalsoType1receptor.IthasbeendesignatedCD121a.TheType2receptorhasbeendesignatedCD121b.ItisisolatedfromB-cells,granulocytes,andmacrophages.ItisexpressedpredominantlyonB-cellsandcellsofthemyelomonocyticlineageandisencodedbyaseparategene.
Description:
RecombinantIL-1betaisadisulfide-linkedmonomerproteinconsistingoftwo154aminoacidresidues.RecombinantIL-1betamigratesasanapproximately17kDaproteinundernon-reducingandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingHumanInterleukin-1betamaturechainwasexpressedinE.coli.
UniProt
P01584
Synonym(s):
IL-1B,IL-1beta,Interleukin-1beta,Catabolin
Purity:
≥97%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BiologicalActivity:
TheED50asdeterminedbythedose-dependentproliferationofmurineD10Scells,wasfoundtobe≤2.0pg/ml,correspondingtoaspecificactivityof≥2x109units/mg
Formulation:
Lyophilizedfrom0.2µmfilteredPBS,pH7.0.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.J.Pharmacol.Exp.Ther.,Oct2009,331:104-113.
2.J.Endocrinol.,Oct2009,203:55-63.
3.Nephrol.Dial.Transplant.,Sep2009,24:2655-2665.
2.J.Endocrinol.,Oct2009,203:55-63.
3.Nephrol.Dial.Transplant.,Sep2009,24:2655-2665.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
