Bpsbioscience/Human Insulin Like Growth Factor-I/90166-A/20 µg
商品编号：
90166-A
品牌：
bpsbioscience
市场价：
¥2600.00
美元价：
1560.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:8kDa
Genbank#:P01343
a.a:49–118
Background:
Insulin-likegrowthfactorI,alsoknownassomatomedinC,isthedominanteffectorofgrowthhormoneandisstructurallyhomologoustoproinsulin.HumanIGF-IissynthesizedastwoprecursorisoformswithN-andalternateC-terminalpropeptides.Theseisoformsaredifferentiallyexpressedbyvarioustissues.The7.6kDamatureIGF-IisidenticalbetweenisoformsandisgeneratedbyproteolyticremovaloftheN-andC-terminalregions.MaturehumanIGF-Ishares94%and96%a.a.sequenceidentitywithmouseandratIGF-I,respectively,andexhibitscross-speciesactivity.Itshares64%aasequenceidentitywithmaturehumanIGF-II.CirculatingIGF-Iisproducedbyhepatocytes,whilelocalIGF-Iisproducedbymanyothertissuesinwhichithasparacrineeffects.
Description:
RecombinantHumanIGF-1isadisulfide-linkedmonomericproteinconsistingof71aminoacidresidues.IGF-1migratesasanapproximately8kDaproteinundernon-reducingandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingHumanIGF-1maturechainwasexpressedinE.coli.
UniProt
P01343
Synonym(s):
Insulin-LikeGrowthFactor1,SomatomedinC,IGF1
Purity:
≥95%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BIOLOGicalActivity:
TheED50wasdeterminedbyacellproliferationassayusingFDC-P1cellsis≤1.0ng/ml,correspondingtoaspecificactivityof≥ 1x106units/mg.
Formulation:
Lyophilizedfrom0.2µmfilteredPBS,pH7.4.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.J.Clin.Endocrinol.Metab.October1,2009.94(10):3913-3921.
2.Mol.Biol.Cell,Sep2009,20:3810-3817.
3.Endocrinology,Sep2009,150:4395-4403.
2.Mol.Biol.Cell,Sep2009,20:3810-3817.
3.Endocrinology,Sep2009,150:4395-4403.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
