Bpsbioscience/人IL-8/90258-A/5µg
商品编号：
90258-A
品牌：
bpsbioscience
市场价：
¥2600.00
美元价：
1560.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:9kDa
Genbank#:P10145
a.a:23–99
Background:
IL-8isproducedbystimulatedmonocytesbutnotbytissuemacrophagesorT-lymphocytes.InmanycelltypesthesynthesisofIL-8isstronglystimulatedbyIL-1andTNF-alpha.Inhumanskinfibroblasts,theexpressionofIL-8isenhancedbyLeukoregulin.TheexpressionofIL-8fromrestingandstimulatedhumanbloodmonocytesisup-regulatedbyIL-7.Inchondrocytes,thesynthesisofIL-8isstimulatedbyIL1-betaTNF-alphaandbacteriallipopolysaccharides.Inhumanastrocytes,thesynthesisandsecretionofIL-8isinducedbyIL-1andTNF-alpha.TheIL-8receptor(CD128)isadimericglycoproteinconsistingofa59kDaanda67kDasubunit.ItisexpressedinmanydifferentcelltypesincludingthosenotrespondingtoIL-8.TheBIOLOGicalactivitiesofIL-8resemblethoseofarelatedprotein,NAP-2.IL-8differsfromallothercytokinesinitsABIlitytospecificallyactivateneutrophilgranulocytes.Inneutrophils,IL-8causesatransientincreaseincytosoliccalciumlevelsandthereleaseofenzymesfromgranules.IL-8alsoenhancesthemetabolismofreactiveoxygenspeciesandincreaseschemotaxisandtheenhancedexpressionofadhesionmolecules.Pre-activationbyIL-3isrequiredtorenderbasophilsandneutrophilssusceptIBLetofurtheractivationbyIL-8.NativehumanIL-8,generatedbytheproteolyticremovalofthesignalpeptideandpropeptide,hasacalculatedmolecularmassofapproximately8kDa.
Description:
RecombinanthumanInterleukin-8isadisulfide-linkedmonomerproteinconsistingof78aminoacidresidues,migratesasanapproximately9kDaproteinundernon-reducingandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingHumanInterleukin-8maturechainwasexpressedinE.coli.
UniProt
P10145
Synonym(s):
IL-8,Interleukin8,CXCL8,TSG-1,IL8
Purity:
≥95%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BiologicalActivity:
Activitywasdeterminedbytheabilitytochemoattracthumanpheripheralbloodmonocyteswasfoundtobeintherangeof25.0-100.0ng/ml.
Formulation:
Lyophilizedfroma0.2µmfilteredPBSsolution,pH7.0
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/Stability:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
Orjalo,A.,etal.2009.Proc.Natl.Acad.Sci.106:17030-5.
2.Stillie,R.M.,etal.J.Leukoc.Biol.,Sep2009,86:529-543.
3.Bautista,M.V.,etal.J.Immunol.,Aug2009,183:2159-2166.
2.Stillie,R.M.,etal.J.Leukoc.Biol.,Sep2009,86:529-543.
3.Bautista,M.V.,etal.J.Immunol.,Aug2009,183:2159-2166.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
AVLPRSAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAENS
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
