Bpsbioscience/Human IGFBP-3/90165-B/25 µg
商品编号：
90165-B
品牌：
bpsbioscience
市场价：
¥4100.00
美元价：
2460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: E. coli
MW: 28 kDa
Genbank #: P17936
Background:
IGFBPs are synthesized in the liver and are produced also by various tumor cell lines and cell types. IGFBPs are high affinity binding proteins for IGF. The major fraction of both types of IGF circulating in the blood are bound non-covalently to these carrier proteins. IGF binding proteins modulate IGF activities by increasing their plasma half lives and by inhibiting or promoting the interactions of IGF with receptors on certain target cells. In addition these binding proteins provide a reservoir for IGF in pericellular spaces. Some IGFBPs also have stimulating effects in vitro and some may inhibit the growth of cells. IGFBP1 is found predominantly in the placenta and the amniotic fluid. The predominant sites of IGFBP1 transcription in the human fetal kidney are those with most active differentiation. Elevated serum levels have been observed in patients with Laron-type dwarfism and Growth hormone deficiency. High serum levels of IGFBP1 are found in newborns and it has been suggested that this could be important in protecting them from hypoglycemia.
Description:
Recombinant IGFBP-3 is a disulfide-linked monomer protein consisting of 264 amino acid residues, migrates as an approximately 28 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human IGFBP-3 mature chain was expressed in E. coli.
UniProt
P17936
Synonym(s):
IGFBP-3, IGF-Binding Protein 3
Purity:
≥95% by SDS-PAGE and HPLC
Endotoxin Level:
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity:
The ED50 was determined by the ability to inhibit IGF-I induced proliferation of MCF-7 and was found to be ≤ 0.5 μg/ml.
Formulation:
Lyophilized from 0.2 µm filtered PBS, pH 7.0.
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:
The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Reference(s):
1. McCarthy, K., et al. Anticancer Research October 2009 vol. 29 no. 10 3785-3790.
2. Volzke, H., et al. Eur J Endocrinol November 1, 2009 161 705-713.
3. Paharkova-Vatchkova, V. And Lee, K-W. Endocr Relat Cancer June 1, 2010 17 293-302.
2. Volzke, H., et al. Eur J Endocrinol November 1, 2009 161 705-713.
3. Paharkova-Vatchkova, V. And Lee, K-W. Endocr Relat Cancer June 1, 2010 17 293-302.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Cytokine/Growth Factors
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
