Bpsbioscience/Human Hepatocyte Growth Factor alpha chain/90157-B/10 µg
商品编号：
90157-B
品牌：
bpsbioscience
市场价：
¥4100.00
美元价：
2460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:54kDa
Genbank#:P14210
a.a:32–484
Background:
Hepatocytegrowthfactor(HGF)isamultifunctionalcytokinethatwasoriginallydescribedasamesenchymal-derivedfactorthatregulatescellgrowth,cellmotility,morphogenesisandangiogenesisthroughactivationofitsreceptor,thetransmembranetyrosinekinaseencodedbythec-Metproto-oncogene.HGFandc-Metareoftenco-expressedorover-expressedinavarietyofhumanmalignanciesincludingglioblastomaandmedullablastoma.ThemultifunctionaleffectsofHGF:c-Metsignalingintumorcellsaremediatedbyanetworkofsignaltransductionpathwaysincludingmitogen-activatedproteinkinase(MAPK)andphosphoinositide3-kinase(PI3K).HGFconsistsoftwosubunitsheldbyadisulfidebond.Thealphasubunit(69kDa)hasalengthof440aminoacids.Thebetasubunit(34kDa)hasalengthof234aminoacids.Thebetachainofthefactorshowsapproximately38%homologyattheproteinleveltotheserineproteasedomainofplasminogen.AvariantofthebetachainhasbeenshowntofunctionasasubunitofahybridcytokineknownasPPBSF[pre-pro-B-cellgrowthstimulatingfactor].HGFisaglycoprotein,thenon-glycosylatedfactorisalsobioactive.
Description:
RecombinantHGFalphaisanon-glycosylateddisulfide-linkedproteinconsistingofthealphachain(463)aminoacidresidues,andmigratesasanapproximately54kDaproteinundernon-reducingandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingHumanHepatocyteGrowthFactoralphachainwasexpressedinE.coli.
UniProt
P14210
Synonym(s):
HGFA,HumanHepatocyteGrowthFactoralphachain,HepapoietinA,ScatterFactor,HPTA
Purity:
≥97%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
Formulation:
Lyophilizedfrom0.2µmfilteredsolutioninTris,pH8.0.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.J.Biol.Chem.,2009,284,28510-28521.
2.AmJPhysiolCellPhysiol,Aug2009,297:C238-C252.
3.Clin.CancerRes.,Jun2009,15:3740-3750.
2.AmJPhysiolCellPhysiol,Aug2009,297:C238-C252.
3.Clin.CancerRes.,Jun2009,15:3740-3750.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPV
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
