Bpsbioscience/Human Granulocyte Macrophage-Colony Stimulating Factor/90145-B/20 µg
商品编号：
90145-B
品牌：
bpsbioscience
市场价：
¥4100.00
美元价：
2460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: E. coli
MW: 15 kDa
Genbank #: P04141
a.a: 18–144
Background:
GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the N-terminal end. The sugar moiety is not required for the full spectrum of biological activities. Non-glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non-glycosylated protein. This protein is secreted together with other factors by T-cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF.
Description:
Recombinant GM-CSF is a disulfide-linked monomer protein, consisting of 127 amino acids and migrates as an approximately 15 kDa protein under reducing conditions. Optimized DNA sequence encoding Human Granulocyte Macrophage-Colony Stimulating Factor mature chain was expressed in E. coli.
UniProt
P04141
Synonym(s):
GM-CSF, CSF2, Granulocyte Macrophage-Colony Stimulating Factor, Colony Stimulating Factor-2, Molgramostin, Sargramostim, CSF
Purity:
≥97% by SDS-PAGE and HPLC
Endotoxin Level:
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity:
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Formulation:
Lyophilized from 0.2 µm filtered PBS solution, pH 7.0.
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:
The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Reference(s):
1. Hiasa, M., et al. Blood November 12, 2009 vol. 114 (20): 4517-4526 .
2. Zhu, S.M., et al. J. Exp. Med. 206 (10): 2141-2149.
3. Chin, P.Y., et al. Hum. Reprod. (2009) 24 (12): 2997-3009.
2. Zhu, S.M., et al. J. Exp. Med. 206 (10): 2141-2149.
3. Chin, P.Y., et al. Hum. Reprod. (2009) 24 (12): 2997-3009.
Warning(s):
Avoid freeze/thaw cycles
Amino Acid Sequence:
APA RSPSPSTQPW EHVNAIQEAR RLLNLSRDTA AEMNETVEVI SEMFDLQEPT CLQTRLELYK QGLRGSLTKL KGPLTMMASH YKQHCPPTPE TSCATQIITF ESFKENLKDF LLVIPFDCWE PVQE
Scientific Category:
Cytokine/Growth Factors
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
