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Bpsbioscience/PCSK9(D374T)-LDLR TR-FRET Assay Kit/72011/384 reactions
Bpsbioscience/PCSK9(D374T)-LDLR TR-FRET Assay Kit/72011/384 reactions
商品编号：
72011
品牌：
bpsbioscience
市场价：
¥14900.00
美元价：
8940.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
PCSK9isacrucialplayerintheregulationofplasmacholesterolhomeostasis.Itbindstotheectodomainofhepaticlow-densitylipidreceptorfamilymembers:lowdensitylipoproteinreceptor(LDLR),verylowdensitylipoproteinreceptor(VLDLR),apolipoproteinEreceptor(LRP1/APOER)andapolipoproteinreceptor2(LRP8/APOER2),andpromotestheirdegradation.PCSK9actsviaanon-proteolyticmechanismtoenhancethedegradationofthehepaticLDLRthroughaclathrinLDLRAP1/ARH-mediatedpathway.MaypreventtherecyclingofLDLRfromendosomestothecellsurfaceordirectittolysosomesfordegradation.TheD374TmutationresultsinhigheraffinityofPCSK9forLDLR.
Description:
ThePCSK9(D374T)-LDLRTR-FRETAssayKitisdesignedtomeasuretheinhibitionofthehighaffinityPCSK9mutant(D374T)bindingtoLDLRinahomogeneous384reactionformat.ThePCSK9AssayKitassayrequiresnotime-consumingwashingsteps,makingitespeciallysuitableforhighthroughputscreeningapplications.Theassayprocedureisstraightforwardandsimple;asamplecontainingeuropium-labeled(Eu)LDLRectodomain,dye-labeledacceptor,biotin-labeledPCSK9(D374T),andaninhibitorisincubatedfortwohours.Then,thefluorescenceintensityismeasuredusingafluorescencereader.
UniProt
Q8NBP7
Synonym(s):
pcsk9,cholesterol,LDL,kexin,PCSK9AssayKit
SuppliedAs:
KitcomesinaconvenientformatwithpurifiedATAD2A,ligands,Tbdonor,dye-labeledacceptor,assaybufferandmicrotiterplatetoperform384reactions.
Format:
COMPONENTS
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
Atleast6monthsfromdateofreceiptwhenstoredasdirected.
Application(s):
GreatforscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
1.Chan,J.C.etal.(2009).Proc.Natl.Acad.Sci.USA,106,9820-9825.
2.Liang,H.,etal.(2012).J.Pharmacol.Exp.Ther.3402289-236.
2.Liang,H.,etal.(2012).J.Pharmacol.Exp.Ther.3402289-236.
Notes:
MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
FluorescentmicroplatereadercapableofmeasuringTimeResolvedFluorescenceResonanceEnergyTransfer(TR-FRET)
Adjustablemicropipettorandsteriletips
ScientificCategory:
CholesterolRelated
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
