Bpsbioscience/IKCA1（KCNN4）-HEK293重组细胞系/90330/2瓶
商品编号：
90330
品牌：
bpsbioscience
市场价：
¥150000.00
美元价：
90000.00
产品分类：
常规细胞株
公司分类：
Routine_cell_strain
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:human
Genbank#:NM_002250
Background:
IKCA1ispartofapotentiallyheterotetramericvoltage-independentpotassiumchannelthatisactivatedbyintracellularcalcium.Activationisfollowedbymembranehyperpolarization,whichpromotescalciuminflux.Theencodedproteinmaybepartofthepredominantcalcium-activatedpotassiumchannelinT-lymphocytes.
Description:
Stable,recombinantHEK293celllineexpressinghumanIKCA1,alsoknownasKCNN4(Intermediateconductancecalcium-activatedpotassiumchannelprotein4),IK1,hKCa4,andhSK4,GenBankAccessionNo.NM_002250.
Synonym(s):
IKCA1,KCNN4,Intermediateconductancecalcium-activatedpotassiumchannelprotein4,IK1,hKCa4,hSK4
SuppliedAs:
Eachvialcontains~2X106cellsin1mlof10%DMSO.
BIOLOGicalActivity:
N-terminalFLAG-taggedhumanIKCA1hasbeenstablyexpressedinHEK293celllineanditsexpressionwasconfirmedbyWesternblotting.FunctionalanalysisofIKCA1channelinthestablerecombinantHEK293wasaccomplishedbyusingthemembranepotential–sensitivefluorescencedyeDiBAC4(3).WhenIKCA1-expressedHEK293cellswerestimulatedwithcalcium-specificionophoreionomycintoraisetheintracellularlevelofCa2+,IKCA1channelwasactivated,leADIngtohyperpolarizationandadecreaseincellfluorescenceinthefunctionalfluorescenceassaywithDiBAC4(3).ThisdecreaseofcellfluorescencewasblockedbytheIKCA1channelinhibitor,clotrimazole(CLT).
HostCellline:
HEK293cells
MycoplasmaTesting:
ThecelllinehasbeenscreenedusingthePCR-basedVenorGeMMycoplasmaDetectionkit(SigmaAldrich)toconfirmtheabsenceofMycoplasmaspecies.
Instructionsforuse:
Seedatasheetfordetailedculturingandassayprotocol.
Storage/StABIlity:
Storeinliquidnitrogenimmediatelyuponreceipt.
Application(s):
•DrugcompoundscreeningforactivatorsorinhibitorsofIKCA1
•FunctionalassaysforIKCA1
•Efficientantigenformouseimmunization
•FunctionalassaysforIKCA1
•Efficientantigenformouseimmunization
Reference(s):
1.GhanshaniSetal.(2000).JBiolChem.275(47):37137-37149.
2.HoffmanJFetal.(2003).PNAS100(12):7366-7371.
3.de-AllieFAetal.(1996).BrJPharmacol.117(3):479-487.
4.TerstappenGCetal.(2003).NeurosciLett.346(1-2):85-88.
2.HoffmanJFetal.(2003).PNAS100(12):7366-7371.
3.de-AllieFAetal.(1996).BrJPharmacol.117(3):479-487.
4.TerstappenGCetal.(2003).NeurosciLett.346(1-2):85-88.
Notes:
Contactusforpricing.
LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermittheuseofthiscelllineinhumansorfortherapeuticordruguse.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
IonChannel
ProductType:
CellLine
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
