Bpsbioscience/TCR Activator / PD-L1 Mammalian Expression Kit/60610/500 reactions -免疫在线蚂蚁淘旗下平台

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商品详细Bpsbioscience/TCR Activator / PD-L1 Mammalian Expression Kit/60610/500 reactions

Bpsbioscience/TCR Activator / PD-L1 Mammalian Expression Kit/60610/500 reactions

商品编号： 60610

品牌： bpsbioscience

市场价： ¥11800.00

美元价： 7080.00

产地：美国(厂家直采)

公司：

产品分类：其它检测试剂盒

公司分类： Other_kits

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Species:Human

Background:

ThebindingofProgrammedCellDeathProtein1(PD-1),areceptorexpressedonactivatedTcells,toitsligands,PD-L1andPD-L2,negativelyregulatesimmuneresponses.ThePD-1ligandsarefoundonmostcancers,andPD-1:PD-L1/2interactioninhibitsTcellactivityandallowscancercellstoescapeimmunesurveillance.ThePD-1:PD-L1/2pathwayisalsoinvolvedinregulatingautoimmuneresponses,makingtheseproteinspromisingtherapeutictargetsforanumberofcancers,aswellasmultiplesclerosis,arthritis,lupus,andtypeIdiabetes.

Description:

TherecombinantexpressionvectorsaredesignedtoexpresshumanengineeredTcellreceptor(TCR)activatorandhumanPD-L1(GenBankAccession#NM_014143)inmammaliancells.ThetransfectedcellscanbeusedinconjunctionwithPD-1/NFATReporter/JurkatTcells(BPS#60535)tostudytheinteractionsofPD-1withPD-L1ligandinacellularcontextandscreenformodulatorsofthissignalingpathway.

Inthisassay,PD-1/NFATReporter/JurkatTcellsareusedaseffectorcells;HEK293cellsoverexpressingPD-L1(orPD-L2)andanengineeredTcellreceptor(TCR)activatorareusedastargetcells.Whenthesetwocellsareco-cultivated,TCRcomplexesoneffectorcellsareactivatedbyTCRactivatorontargetcells,resultinginexpressionoftheNFATluciferasereporter.However,PD1andPD-L1(orPD-L2)ligationpreventsTCRactivationandsuppressestheNFAT-responsiveluciferaseactivity.Thisinhibitioncanbespecificallyreversedbyanti-PD1oranti-PD-L1antibodies.PD1/PD-L1neutralizingantibodiesblockPD1:PD-L1interactionandpromoteTcellactivation,resultinginreactivationoftheNFATresponsiveluciferasereporter.

MaterialsRequiredButNotProvided:

•HEK293cellanditsgrowthmediumorothercelllines
•Transfectionreagentformammaliancellline[BPSusesLipofectamine™2000(lifetechnologies#11668027).However,othertransfectionreagentsworkequallywell.]
•PD-1/NFATReporterJurkatTcells(BPSCat.#60535)
•Opti-MEMIReducedSerumMedium(lifetechnologies#31985-062)
•Assaymedium:RPMI1640+10%FBS+1%Penicillin/Streptomycin
•Anti-PD-1neutralizingantibody(BPSCat.#71120)
•Anti-PD-L1neutralizingantibody(BPSCat.#71213)
•96-welltissueculture-treatedwhiteclear-bottomassayplate
•One-Stepluciferaseassaysystem(BPSCat.#60690)orotherluciferasereagentsformeasuringfireflyluciferaseactivity•Luminometer

Synonym(s):

ProgrammedCellDeath1Ligand1,PDL1,PD-L1,B7homolog1,B7H1,B7-H1,CD274,PDCD1L1

Format:

Components

Instructionsforuse:

Seeassaykitprotocolfordetailedinstructions.

Storage/StABIlity:

Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.

Application(s):

•ScreenforactivatorsorinhibitorsofPD-1signalinginacellularcontext•CharacterizetheBIOLOGicalactivityofPD-1anditsinteractionswithligands

Notes:

MaterialsRequiredbutNotSupplied
•HEK293cellanditsgrowthmediumorothercelllines
•Transfectionreagentformammaliancellline[WeuseLipofectamine™2000(lifetechnologies#11668027).However,othertransfectionreagentsworkequallywell.]•PD-1/NFATReporterJurkatTcells(BPS#60535)•Opti-MEMIReducedSerumMedium(lifetechnologies#31985-062)
•Assaymedium:RPMI1640+10%FBS+1%Penicillin/Streptomycin
•Anti-PD-1neutralizingantibody:BPSbioscience#71120
•Anti-PD-L1neutralizingantibody:BPSbioscience#71213
•96-welltissueculture-treatedwhiteclear-bottomassayplate
•One-Stepluciferaseassaysystem(BPSbioscience#60690)orotherluciferasereagentsformeasuringfireflyluciferaseactivity•Luminometer

Warning(s):

Avoidfreeze/thawcycles.

ScientificCategory:

Immunotherapy

ProductType:

AssayKit

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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