Bpsbioscience/PCSK9, C-terminal Avi-His, Biotin-labeled/71305/50 µg
商品编号：
71305
品牌：
bpsbioscience
市场价：
¥8100.00
美元价：
4860.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:HEK293cells
MW:73.8kDa
Genbank#:NM_174936
Tag(s):C-terminalHis,AviTags
a.a:31–692(end)
Background:
PCSK9isacrucialplayerintheregulationofplasmacholesterolhomeostasis.Itbindstolow-densitylipidreceptorfamilymembers:lowdensitylipoproteinreceptor(LDLR),verylowdensitylipoproteinreceptor(VLDLR),apolipoproteinEreceptor(LRP1/APOER)andapolipoproteinreceptor2(LRP8/APOER2),andpromotestheirdegradation.Actsviaanon-proteolyticmechanismtoenhancethedegradationofthehepaticLDLRthroughaclathrinLDLRAP1/ARH-mediatedpathway.MaypreventtherecyclingofLDLRfromendosomestothecellsurfaceordirectittolysosomesfordegradation.CaninduceubiquitinationofLDLRleADIngtoitssubsequentdegradation.InhibitsintracellulardegradationofAPOBviatheautophagosome/lysosomepathwayinaLDLR-independentmanner.Involvedinthedisposalofnon-acetylatedintermediatesofBACE1intheearlysecretorypathway.InhibitsepithelialNa(+)channel(ENaC)-mediatedNa(+)absorptionbyreducingENaCsurfaceexpressionprimarilybyincreasingitsproteasomaldegradation.RegulatesneuronalapoptosisviamodulationofLRP8/APOER2levelsandrelatedanti-apoptoticsignalingpathways.
Description:
Humanproproteinconvertasesubtilisin/kexintype9(PCSK9),alsoknownasFH3,HCHOLA3,andPC9,GenBankAccessionNo.NM_174936,a.a.31-692(end),withC-terminalHisandAvitags,MW=73.8kDa(calculated),expressedinanHEK293cellexpressionsystemandenzymaticallybiotinylatedusingAvitag™technology.
UniProt
Q8NBP7
Synonym(s):
BiotinylatedPCSK9,FH3,HCHOLA3,PC9,NARC1,NeuralApoptosisRegulatedConvertase1
AssayConditions:
50µLreactionmix(50mMHEPESpH7.4,50mMNaCl,200µMCaCl2,0.05%Tween-20,0.1%BSA,and0–1500ngbiotin-labeledPCSK9)isaddedtowellsprecoatedwithLDLREctodomain.Incubatefor2hr.Addstreptavidin-labeledHRPandincubate1hour.Finally,addHRPchemiluminescentsubstratesandreadluminescence.
Formulation:
40mMTris,pH8.0,110mMNaCl,2.2mMKCl,and20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforstudyingproteinbindingandscreeningsmallmoleculesfordrugdiscovery.
Reference(s):
1.Abifadel,M.,etal.,NatGenet.2003Jun,34(2):154-6.
2.Abifadel,M., etal.,HumMutat. 2009Apr;30(4):520-9.
Notes:
PCSK9isautocleavedtothe~14kDaprodomain(a.a.31-152)andthe~60kDamatureform(a.a.153-692(end)),whichrunathigherapparentMWbySDS-PAGE.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
CholesterolRelated
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
