Bpsbioscience/PCSK9(D374T), Biotin-labeled/71211/20 µg
商品编号：
71211
品牌：
bpsbioscience
市场价：
¥9500.00
美元价：
5700.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:HEK293Cells
MW:74kDa
Genbank#:NM_174936
Tag(s):C-terminalHis,Avi-tags
a.a:31-692(end)
Background:
PCSK9isacrucialplayerintheregulationofplasmacholesterolhomeostasis.Itbindstotheectodomainofhepaticlow-densitylipidreceptorfamilymembers:lowdensitylipoproteinreceptor(LDLR),verylowdensitylipoproteinreceptor(VLDLR),apolipoproteinEreceptor(LRP1/APOER)andapolipoproteinreceptor2(LRP8/APOER2),andpromotestheirdegradation.PCSK9actsviaanon-proteolyticmechanismtoenhancethedegradationofthehepaticLDLRthroughaclathrinLDLRAP1/ARH-mediatedpathway.MaypreventtherecyclingofLDLRfromendosomestothecellsurfaceordirectittolysosomesfordegradation.TheD374TmutationresultsinhigheraffinityofPCSK9forLDLR.
Description:
Humanproproteinconvertasesubtilisin/kexintype9(PCSK9),alsoknownasFH3,HCHOLA3,andPC9,GenBankAccessionNo.NM_174936,a.a.31-692(end),withC-terminalHis-andAvi-tagsandaD374Tmutation,MW=73.8kDa,expressedinanHEK293cellexpressionsystemandenzymaticallybiotinylatedusingAvitag™technology.PCSK9isautocleavedtothe~14kDaprodomain(a.a.31-152)andthe~60kDamatureform(a.a.153-692),whichrunathigherMWbySDS-PAGE.
UniProt
Q8NBP7
Synonym(s):
Proproteinconvertasesubtilisin/kexintype9,FH3,HCHOLA3,PC9
AssayConditions:
ReactionwasperformedusingPCSK9(D374T)-LDLRTR-FRETAssayKit(Cat.#72011).Briefly,5ngofeuropium-labeledLDLRweremixedwithvaryingamountsofPCSK9(D374T)anddyelabeledacceptorinPCSK9TR-FRETassaybufferandincubatedatRTfor2hr.Donoremissionwasreadat620nmfollowedbyacceptoremissionat665nm.
Formulation:
40mMTris-HClpH8.0,110mMNaCl,2.2mMKCl,and20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,bindingstudies,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Chan,J.C.etal.(2009).Proc.Natl.Acad.Sci.USA,106:9820-9825.
2.Liang,H.,etal.(2012).J.Pharmacol.Exp.Ther.340:2289-236.
2.Liang,H.,etal.(2012).J.Pharmacol.Exp.Ther.340:2289-236.
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
ScientificCategory:
CholesterolRelated
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
