Bpsbioscience/LDLR, Biotin-labeled/71206/20 µg
商品编号：
71206
品牌：
bpsbioscience
市场价：
¥9500.00
美元价：
5700.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:HEK293cells
MW:87.7kDa
Genbank#:NM_000527
Tag(s):C-terminalFLAGandAvitags
a.a:22–788(end)
Background:
Thelowdensitylipoproteinreceptor(LDLR)genefamilyconsistsofcellsurfaceproteinsinvolvedinreceptor-mediatedendocytosisofspecificligands.Lowdensitylipoprotein(LDL)isnormallyboundatthecellmembraneandtakenintothecellendingupinlysosomeswheretheproteinisdegradedandthecholesterolismadeavailableforrepressionofmicrosomalenzyme3-hydroxy-3-methylglutarylcoenzymeA(HMGCoA)reductase,therate-limitingstepincholesterolsynthesis.Atthesametime,areciprocalstimulationofcholesterolestersynthesistakesplace.Mutationsinthisgenecausetheautosomaldominantdisorder,familialhypercholesterolemia.IncaseofHIV-1infection,itfunctionsasareceptorforextracellularTatinneurons,mediatingitsinternalizationinuninfectedcells.PCSK9bindstotheepidermalgrowthfactor-likerepeatA(EGF-A)domainofLDLR,inducingLDLRdegradation.
Description:
Humanlowdensitylipoproteinreceptor(LDLR),alsoknownasFH,FHC,andLDLCQ2,GenBankAccessionNo.NM_000527,a.a.22-788(end),withC-terminalFLAGandAvitags,MW=87.7kDa*,expressedinanHEK293cellexpressionsystemandenzymaticallybiotinylatedusingAvitag™technology.
UniProt
P01130
Synonym(s):
FH,FHC,LDLR,LDLCQ2,lowdensitylipoproteinreceptor
AssayConditions:
Coataplatewith100ngofPCSK9.Adda25µLreactionmix(50mMHEPESpH7.4,50mMNaCl,200µMCaCl2,0.05%Tween,0.1%BSA,andupto200ngLDLR-biotin)tothecoatedwellsandincubatefor2hr.AddStreptavidin-HRPlabeledantibodyandincubate1hr.Finally,addHRPchemiluminescentsubstratesandreadluminescence.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforstudyingproteinbindingandscreeningsmallmoleculesfordrugdiscovery.
Reference(s):
1.Holla,L.,etal.,BMCCellBiol.2007Mar1,8:9.
2.Qian,YW.,etal.,JLipidRes.2007Jul,48(7):1488-98.
3.Fasano,T.,etal.,Athersclerosis.2009Mar,203(1):166-71.
2.Qian,YW.,etal.,JLipidRes.2007Jul,48(7):1488-98.
3.Fasano,T.,etal.,Athersclerosis.2009Mar,203(1):166-71.
Notes:
*LDLRisheavilyglycosylated,resultinginhighermolecularweight.Thetwobandsshowncorrespondtodifferingstatesofglycosylation.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Cholesterol-RelatedProteins
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
