Bpsbioscience/Histone Octamer, Biotin-labeled, His-tag/52061/50 µg
商品编号：
52061
品牌：
bpsbioscience
市场价：
¥9300.00
美元价：
5580.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:117kDa
Genbank#:NM_033445,NM_003528,NM_003532,andNM_003548
Tag(s):N-terminalHis-tag,Biotin
Background:
ThehistoneoctameriscomposedofacentralheterotetrameroftwocopiesofbothhistonesH3andH4,flankedbytwoheterodimersofhistonesH2AandH2B.TheoctamerassembleswhentheH3/H4tetramercomplexeswithtwoH2A/H2Bdimers.ThesehistonesofthehistoneoctamerallcontainN-terminaltailsthatemanatefromtheircentralhistonefoldsandaretargetsforepigeneticmodification.
Description:
Humanrecombinanthistoneoctamerconsistingof2moleculeseachofN-terminalHis-taggedhistonesH2A(a.a.2-130(end)),H2B(a.a.2-126(end)),H3(a.a.2-137(end)),andH4(a.a.2-103(end)),expressedinanE.coliexpressionsystem.GenBankAccessionNos.areNM_033445,NM_003528,NM_003532,andNM_003548,respectively.H2Bhasbeenbiotin-labeledviathiolmodificationofanadditionalC-terminalcysteinepriortoassembly.GlobalMW=117kDa.
UniProt
Q7L7L0,Q16778,P68431,P62805
Formulation:
45mMTrispH7.5,2MNaCl,and10%glycerol.
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Substrateforhistonemethyltransferaseoracetyltransferaseassays.IdealforscreeningsmallmolecularinhibitorsofhistonemethyltransferasesandacetyltransferasesfordrugdiscoveryandHTSapplications.
Reference(s):
1.MillerKM,JacksonSP.BiochemSocTrans40(2):370-6(2012).
2.RautVV,PandeySM,SainisJK.AnnBot108(7):1235-46(2011).
3.ParkK,FasmanGD.Biochemistry.1987Dec15;26(25):8042-5.
4.Meagher,R.B.,Mussar,K.J.,EpigeneticsChromatin2012Jul20,5(1):11.
Notes:
SincetherecombinanthistoneproteinsinthisoctamerwerepreparedinE.coli,theywillnothavemethyl,acetylormanyotherepigeneticmarks.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Methyltransferase
ProductType:
Substrate
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
