Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human PHF8 (Cat. # 25308), crude serum and flow-through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,500.

Western blot analysis using the antibody directed against PHF8
E14TG2A mouse embryonic stem cells were transfected with a conditional allele of PHF8. Nuclear extracts (135 µg) from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analyzed by Western blot using the antibody against PHF8 (Cat. # 25308). The antibody was diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Western blot analysis was performed by Philipp Seibert, BIOTEC, Dept. of Genomics, TU Dresden, Tatzberg 47/49, 01307 Dresden. 

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