Bpsbioscience/Anti–PADI4 polyclonal antibody/25305/100 µl-免疫在线蚂蚁淘旗下平台

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商品详细Bpsbioscience/Anti–PADI4 polyclonal antibody/25305/100 µl

Bpsbioscience/Anti–PADI4 polyclonal antibody/25305/100 µl

商品编号： 25305

品牌： bpsbioscience

市场价： ¥5800.00

美元价： 3480.00

产地：美国(厂家直采)

公司：

产品分类：多克隆抗体

公司分类： Polyclonal_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

HostSpecies:Rabbit

Clonality:polyclonal

Background:

Proteinargininedeiminasescatalysetheconversionofarginineresiduesinproteinstocitrulline.PADI4(UniProt/Swiss–ProtentryQ9UM07)catalysesthecitrullination-deiminationofArg-8andArg-17fromhistoneH3andArg-3fromhistoneH4.ThispreventstheirmethylationbyCARM1andHRMT1L2/PRMT1,therebyrepressingtranscription.PADI4alsocitrullinatesEP300/P300atArg-2142,whichfavoursitsinteractionwithNCOA2/GRIP.

Description:

PolyclonalantibodyraisedinrabbitagainsthumanPADI4(Protein–argininedeiminasetype–4),usingaKLH–conjugatedsyntheticpeptidecontainingasequencefromtheN–terminusoftheprotein

Synonym(s):

PAD,HL–60PAD,PDI4,PADI5,PDI5,Pad

AssayConditions:

Determinationoftheantibodytiter
Todeterminethetiteroftheantibody,anELISAwasperformedusingaserialdilutionofantibodydirectedagainstPADI4(Cat.No.25305).Thewellswerecoatedwiththepeptideusedforimmunisationoftherabbit.Byplottingtheabsorbanceagainsttheantibodydilution(Figure1),thetiteroftheantibodywasestimatedtobe1:40,200.

WesternblotanalysisusingtheantibodydirectedagainstPADI4
WesternblotwasperformedonnuclearextractsfromHeLacells(HeLaNE,40μg)withtheantibodyagainstPADI4(Cat.No.25305),diluted1:1,000inTBS-Tweencontaining5%skimmedmilk(Figure2).ThemolecularweightMarker(inkDa)isshownontheleft;thelocationoftheproteinofinterestisindicatedontheright."

Concentration:

100µl

Formulation:

Wholeantiserumfromrabbitcontaining0.05%azide

SpeciesReactivity:

Human

Purification:

Wholeserum

Immunogen:

syntheticpeptide

Format:

aqueoussolution

Storage/StABIlity:

Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.

Application(s):

ELISA(1:1000–1:5000)
WB(1:1000)

Notes:

Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.

Warning(s):

Avoidfreeze/thawcycles

ScientificCategory:

Antibodies

ProductType:

Antibody

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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