WesternblotanalysisusingtheantibodydirectedagainstL3MBTL1
WesternblotwasperformedonnuclearextractsfromHeLacells(HeLaNE)usingtheantibodyagainstL3MBTL1(Cat.#25301)diluted1:1,000inTBS-Tweencontaining5%skimmedmilk(lane2).AmolecularweightMarkerisshowninlane1;thelocationoftheproteinofinterestisindicatedontheright.
ImmunoprecipitationusingtheantibodydirectedagainstL3MBTL1
ImmunoprecipitationwasperformedonnuclearextractsfromcellstransfectedwithFLAG-taggedL3MBTL1(L3MBTL1-F)withtheantibodyagainstL3MBTL1(Cat.#25301)andwithanIgGnegativecontrolantibody.Westernblotwasperformedwithananti-FLAGantibodytodemonstratethepresenceofL3MBTL1-Fintheinput(lane1),intheprecipitatedfraction(P)andinthesupernatant(SN).ThesedatashowthatL3MBTL1-FwasefficientlyprecipitatedbytheL3MBTL1antibody(lane3and5),whereasitwasnotprecipitatedbytheIgGnegativecontrolantibody(lane2and4).
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.
IP(1–5µg/IP)