Bpsbioscience/Anti–Jarid1b polyclonal antibody/25293/100 µl-免疫在线蚂蚁淘旗下平台

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商品详细Bpsbioscience/Anti–Jarid1b polyclonal antibody/25293/100 µl

Bpsbioscience/Anti–Jarid1b polyclonal antibody/25293/100 µl

商品编号： 25293

品牌： bpsbioscience

市场价： ¥5800.00

美元价： 3480.00

产地：美国(厂家直采)

公司：

产品分类：多克隆抗体

公司分类： Polyclonal_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

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商品介绍

HostSpecies:Rabbit

Clonality:polyclonal

Background:

JARID1B(UniProt/Swiss–ProtentryQ9UGL1)isahistonedemethylasewhichspecificallydemethylatesmono–,di,andtrimethylatedlysine4ofhistoneH3.ItactsasatranscriptionalrepressorforthetumorsuppressorgenesBRCA1andHOXA5andhasbeenassociatedwithbreastcancerdevelopment.Incontrast,inmelanomaJARID1Bitselfmayfunctionasatumorsuppressor.

Description:

PolyclonalantibodyraisedinrabbitagainstmouseJarid1b(Jumonji,ATrichinteractivedomain1B),usingaKLH–conjugatedsyntheticpeptidescontaininganaminoacidsequencefromthecentralpartoftheprotein.

Synonym(s):

KDM5B,CT31,PLU1,PUT1,RBBP2H1,RBP2–like

AssayConditions:

Determinationofthetiter
Todeterminethetiter,anELISAwasperformedusingaserialdilutionoftheantibodydirectedagainstmouseJarid1b(Cat.#25293).Theplateswerecoatedwiththepeptideusedforimmunizationoftherabbit.Byplottingtheabsorbanceagainsttheantibodydilution(Figure1),thetiteroftheantibodywasestimatedtobe1:2,200.

WesternblotanalysisusingtheantibodydirectedagainstJarid1b
Westernblotwasperformedonwholecelllysatesfrommousefibroblasts(NIH3T3)andembryonicstemcells(E14Tg2a)withtheantibodyagainstmouseJarid1b(Cat.#25293),diluted1:500inBSA/PBS-Tween.ThemolecularweightMarker(inkDa)isshownontheleft;thelocationoftheproteinofinterest(expectedsize:179kDa)isindicatedontheright.

Concentration:

100µl

Formulation:

Wholeantiserumfromrabbitcontaining0.05%azide

SpeciesReactivity:

Mouse

Purification:

Wholeserum

Immunogen:

syntheticpeptide

Format:

aqueoussolution

Storage/StABIlity:

Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.

Application(s):

ELISA(1:100–1:200)
WB(1:500)

Notes:

Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.

Warning(s):

Avoidfreeze/thawcycles

ScientificCategory:

Antibodies

ProductType:

Antibody

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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