ChIPresultsobtainedwiththemonoclonalantibodydirectedagainstHDAC1
ChIPassayswereperformedusinghumanHeLacells,themonoclonalantibodyagainstHDAC1(Cat.#25286)andoptimizedPCRprimersetsforqPCR.ChIPwasperformedonshearedchromatinfrom10,000cells.Atitrationoftheantibodyconsistingof1,2,5,and10µgperChIPexperimentwasanalyzed.IgG(5µg/IP)wasusedasnegativeIPcontrol.QPCRwasperformedwithprimersfortheGAPDHpromoterandforthecodingregionofp21,aknowntargetgeneofHDAC1.Figure1showstherecovery,expressedasa%ofinput(therelativeamountofimmunoprecipitatedDNAcomparedtoinputDNAafterqPCRanalysis).

WesternblotanalysisusingthemonoclonalantibodydirectedagainstHDAC1
NuclearextractsfromHeLacells(40µg)wereanalyzedbyWesternblotusingthemonoclonalantibodyagainstHDAC1(Cat.#25286)diluted1:2,000inTBS-Tweencontaining5%skimmedmilk.Thepositionoftheproteinofinterestisindicatedontheright(expectedsize:55kDa);theMarker(inkDa)isshownontheleft.

ImmunofluorescenceusingthemonoclonalantibodydirectedagainstHDAC1
HeLacellswerestainedwiththeantibodyagainstHDAC1(Cat.#25286)andwithDAPI.Cellswerefixedwith4%formaldehydefor10min.andblockedwithPBS/TX-100containing5%normalgoatserumand1%BSA.ThecellswereimmunofluorescentlylabelledwiththeHDAC1antibody(left)diluted1:500inblockingsolutionfollowedbyananti-mouseantibodyconjugatedtoAlexa594.ThemiddlepanelshowsstainingofthenucleiwithDAPI.Amergeofthetwostainingsisshownontheright.

Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.