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4000-520-616
当前位置: 首页 > 产品中心 > Polyclonal_antibody > Bpsbioscience/Anti–H3K9ac polyclonal antibody/25266/50 µg
商品详细Bpsbioscience/Anti–H3K9ac polyclonal antibody/25266/50 µg
Bpsbioscience/Anti–H3K9ac polyclonal antibody/25266/50 µg
Bpsbioscience/Anti–H3K9ac polyclonal antibody/25266/50 µg
商品编号: 25266
品牌: bpsbioscience
市场价: ¥7500.00
美元价: 4500.00
产地: 美国(厂家直采)
公司:
产品分类: 多克隆抗体
公司分类: Polyclonal_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
HostSpecies:Rabbit
Clonality:polyclonal
Description:
PolyclonalantibodyraisedinrabbitagainsthistoneH3,acetylatedatlysine9(H3K9ac),usingaKLH–conjugatedsyntheticpeptide
AssayConditions:

ChIP-seqresultsobtainedwiththeantibodydirectedagainstH3K9ac
ChIPwasperformedasdescribedaboveusing1μgoftheantibodyagainstH3K9ac(cat.No.25266).TheimmunoprecipitatedDNAwassubsequentlyanalysedwithanIlluminaGenomeAnalyzer.Librarypreparation,clustergenerationandsequencingwereperformedaccordingtothemanufacturer’sinstructions.The36bptagswerealignedtothehumangenomeusingtheELANDalgorithm.Figure2showsthepeakdistributionalongthecompletesequenceanda800kbregionoftheX-chromosome(figure2AandB)andin100kbregionssurroundingtheGAPDHandc-fosgenes(figure2CandD).TheseresultsclearlyshowanenrichmentofH3K9acatthepromotersofactivegenes.

ChIPresultsobtainedwiththeantibodydirectedagainstH3K9ac
ChIPassayswereperformedusingHeLacells,theantibodyagainstH3K9ac(cat.No.25266)andoptimizedPCRprimersetsforqPCR.ChIPwasperformedusingshearedchromatinfrom1millioncells.Atitrationoftheantibodyconsistingof1,2,5and10μgperChIPexperimentwasanalysed.IgG(2μg/IP)wasusedasnegativeIPcontrol.QuantitativePCRwasperformedwithprimersforthepromoteroftheactivec-fosandGAPDHgenes,usedaspositivecontrols,andforthecodingregionoftheinactiveMBgeneandtheSat2satelliterepeat,usedasnegativecontrols.Figure1showstherecovery,expressedasa%ofinput(therelativeamountofimmunoprecipitatedDNAcomparedtoinputDNAafterqPCRanalysis).TheseresultsareinaccordancewiththeobservationthatH3K9acetylationisenrichedatthepromotersofactivegenes.

Determinationoftheantibodytiter
Todeterminethetiteroftheantibody,anELISAwasperformedusingaserialdilutionoftheantibodydirectedagainstH3K9ac(cat.No.25266)inantigencoatedwells.Theantigenusedwasapeptidecontainingthehistonemodificationofinterest.Byplottingtheabsorbanceagainsttheantibodydilution(Figure3),thetiteroftheantibodywasestimatedtobe1:59,600.

CrossreactivitytestusingtheantibodydirectedagainstH3K9ac
ADotBlotanalysiswasperformedtotestthecrossreactivityoftheantibodyagainstH3K9ac(cat.No.25266)withpeptidescontainingotherhistonemodificationsandtheunmodifiedH3K9sequence.Onehundredto0.2pmoloftherespectivepeptideswerespottedonamembrane.Theantibodywasusedatadilutionof1:20,000.Figure4showsahighspecificityoftheantibodyforthemodificationofinterest.ThisantibodyrecognizestheH3K9acetylation,bothinthepresenceandtheabsenceoftheK14acetylationortheS10phosphorylation.

WesternblotanalysisusingtheantibodydirectedagainstH3K9ac
HistoneextractsofHeLacells(15μg)wereanalysedbyWesternblotusingtheantibodyagainstH3K9ac(cat.No.25266)diluted1:1,000inTBS-Tweencontaining5%skimmedmilk.Thepositionoftheproteinofinterestisindicatedontheright;theMarker(inkDa)isshownontheleft.

Concentration:
50µg/47µl
Formulation:
PBScontaining0.05%azideand0.05%ProClin300
SpeciesReactivity:
Human
Purification:
Affinitypurified
Immunogen:
syntheticpeptide
Format:
Aqueousbuffersolution
Storage/StABIlity:

Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.

Application(s):
ChIP/ChIP–seq*(1–2µg/ChIP)
ELISA(1:1000)
DB(1:20,000)
WB(1:750)
Notes:
TheoptimalantibodyamountperIPshouldbedeterminedbytheend–user.Werecommendtesting1–5µgperIP
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Antibodies
ProductType:
Antibody
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。