Bpsbioscience/XIAP，标志标签/80401/20µg
商品编号：
80401
品牌：
bpsbioscience
市场价：
¥7900.00
美元价：
4740.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 57 kDa
Genbank #: NM_001167
Tag(s): N-terminal FLAG-tag
a.a: 2–end
Description:
Human X-linked inhibitor of apoptosis (XIAP), also known as IAP-like protein, API3, X-linked IAP, and BIRC4 (GenBank Accession No. NM_001167), a.a. 2-end, with N-terminal FLAG-tag, MW= 57 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
P98170
Synonym(s):
X-linked inhibitor of apoptosis, XIAP, IAP-like protein, API3, X-linked IAP, BIRC4
Assay Conditions:
Ligase Assay Conditions:
50 µl of reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and XIAP (0-1.25 µg) in 50 mM Tris pH 7.4) is incubated for 30 min at 37°C. Transfer the reaction mixture to a streptavidin-coated plate and incubate for 30 min at 37°C. Add antibody against XIAP, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
Bir Domain Assay Conditions:
50 mM Tris-HCl, pH 7.4, 25 mM NaCl, 0.05% Tween-20, 0.01% BSA, 0.5 nM FAM-SMAC peptide. Incubate 30 minutes at room temperature. Fluorescence polarization is measured at 475 nm/528 nm of exc/em wavelength.
50 µl of reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and XIAP (0-1.25 µg) in 50 mM Tris pH 7.4) is incubated for 30 min at 37°C. Transfer the reaction mixture to a streptavidin-coated plate and incubate for 30 min at 37°C. Add antibody against XIAP, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
Bir Domain Assay Conditions:
50 mM Tris-HCl, pH 7.4, 25 mM NaCl, 0.05% Tween-20, 0.01% BSA, 0.5 nM FAM-SMAC peptide. Incubate 30 minutes at room temperature. Fluorescence polarization is measured at 475 nm/528 nm of exc/em wavelength.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 μg/ml FLAG peptide, 20% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful as an Ubiquitin ligase in conjunction with E1 and E2 for the study of enzyme kinetics. Ideal for Bir Domain binding and inhibition studies of Caspases 3 7, and 9. Also useful for screening inhibitors, and selectivity profiling.
Reference(s):
1. Gu L. et al., Cancer Cell 15 (5), 363-375 (2009).
2. Deveraux Q.L. et al., Nature 388 (6639), 300-304 (1997).
2. Deveraux Q.L. et al., Nature 388 (6639), 300-304 (1997).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Ubiquitin E3 ligase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
