CHO-K1celllineexpressingfulllengthhumanCD27(TNFRSF7).ExpressionisconfirmedbyrealtimeqPCR,Westernblot,andflowcytometry.
ScreeningforantibodiesrecognizingCD27andscreeningantibodiesforbindingaffinities.Since
CHO-K1cellsdonotexpressendogenousCD27,thiscelllineservesasanexcellentmodelfor
studyingchangesinCD27-mediatedsignalingpathways,suchasNF-B,MAPKand
SAPK/JNK.
2.YamamotoHet.al.(1998)NF-BactivationinCD27signaling:InvolvementofTNFreceptor-associatedfactorinitssignalingandidentificationoffunctionalregionofCD27.J.Immunology161:4753-4759
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