Bpsbioscience/Hrd1，相扑His标签/81051/25µg
商品编号：
81051
品牌：
bpsbioscience
市场价：
¥5300.00
美元价：
3180.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:39.3kDa(withoutSUMOtag)
Genbank#:Q86TM6.2
Tag(s):N-terminalSUMO-His-tags
a.a:236–617
Background:
Hrd1isaRINGdomainE3ligasethatisinvolvedintheconjugationofubiquitintotargetsubstrates.Hrd1hasbeendemonstratedtofunctionwiththeE2enzymesUBE2D3(UbcH5c)andUBE2G2invitro.Hrd1isanER-associatedligaseinvolvedinERADandhasbeenlinkedtorheumatoidarthritis.Itispresentinhumanrheumatoidsynovialcellsandfoundtobeacausativefactorforarthropathyininvivostudies.Hrd1isalsofoundinbrainneuronsanditsproperregulationmaybelinkedtoneurodegenerativediseases.ThisconstructisaHrd1N-terminaldeletioncontainingresidues246-617consistingofthecompleteC-terminalcytoplasmicportion.TheN-terminaltransmembranespanningportionhasbeendeleted.ThisHrd1N-terminaldeletionmutantisactiveasanE3ligasecontainingtheRINGandsubstraterecognitiondomains.
Description:
CytoplasmicregionofhumanHrd1recombinantprotein(GenbankAccessionNo.),a.a.236-17,withN-terminalSUMO-tag,expressedinE.coli.
UniProt
Q08109
Synonym(s):
E3ubiquitin-proteinligasesynoviolin,HRD1,KIAA1810,MGC40372,Synovialapoptosisinhibitor1
Purity:
≥85%bySDS-PAGE
BIOLOGicalActivity:
Typicalenzymeconcentrationof20-1000nMisusedforinvitroconjugationdependingonassayconditions.
Formulation:
PBS,10%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
Stablefor≥1yearat–80°C.
Application(s):
UsefulasanE3ligaseinubiquitin-conjugatingreactionsandtoanalyzeupregulation/downregulationofE3enzymes.
Reference(s):
1.Kaneko,M.,etal.Molecularapproachestothetreatment,prophylaxis,anddiagnosisofAlzheimer"sdisease:possIBLeinvolvementofHRD1,anovelmoleculerelatedtoendoplasmicreticulumstress,inAlzheimer"sdisease.J.Pharmacol.Sci,2012.118(3):325-30.
2.Yagishita,N.,etal.Synoviolin,proteinfoldingandthemaintenanceofjointhomeostasis,Nat.Clin.Prac.Rheumatol,2008.4(2):91-7.
3.Yamasaki,S.,etal.Therolesofsynoviolinincrosstalkbetweenendoplasmicreticulumstress-inducedapoptosisandp53pathway.CellCycle6(11):1319-23.
2.Yagishita,N.,etal.Synoviolin,proteinfoldingandthemaintenanceofjointhomeostasis,Nat.Clin.Prac.Rheumatol,2008.4(2):91-7.
3.Yamasaki,S.,etal.Therolesofsynoviolinincrosstalkbetweenendoplasmicreticulumstress-inducedapoptosisandp53pathway.CellCycle6(11):1319-23.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Ubiquitination(UbiquitinLigase(E3))
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
