Bpsbioscience/CAR2，相扑His标签/81050/25µg
商品编号：
81050
品牌：
bpsbioscience
市场价：
¥8400.00
美元价：
5040.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:41kDa
Genbank#:NP_476519
Tag(s):N-terminalSUMO-His-tags
a.a:fulllength
Background:
CARP2(caspase8/10associatedRINGprotein2)isaRING-domainE3(ubiquitinproteinligase)thatisinvolvedintheconjugationofubiquitintotargetsubstrates,alongwithE1andE2enzymes.CARPs(CARP1andCARP2,77%identity)alsobelongtotheIAPfamily(inhibitorsofapoptosisproteins)inhibitingactivationofDED(deatheffectordomain)containingcaspaseproteins(8and10).Inadditiontotargetingcaspases8and10,CARPshavebeenshowntotargetphosphorylatedp53fordegradationinanHdm2independentmanner.FurThermore,CARP2hasbeenshowntobeanegativeregulatorofTNFinducedNF-kappaBactivationbytargetingRIPfordegradation.
Description:
HumanCARP2recombinantprotein(GenbankAccessionNo.NP_476519),fulllengthwithN-terminalSUMO-andHis-tags,expressedinE.coli.
UniProt
O75493
Synonym(s):
RFFL,ringfingerandFYVE-likedomaincontaining1
Purity:
≥95%bySDS-PAGE
BIOLOGicalActivity:
Typicalenzymeconcentrationof100nM-5mMisusedforinvitroconjugation,dependingonconditions.
Formulation:
50mMTrisBuffer,pH7.6,150mMNaCl,10%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
Stablefor≥1yearat–80°C.
Application(s):
UsefulasanE3ligaseinubiquitin-conjugatingreactionsandtoanalyzeupregulation/downregulationofE3enzymes.
Reference(s):
1.Yang,W.,etal.,CARPsareubiquitinligasesthatpromoteMDM2-independentp53andphospho-p53ser20degradation.JBiolChem,2007.282:3273-81.
2.Yang,W.andW.S.El-Deiry,CARPsareE3ligasesthattargetapicalcaspasesandp53.CancerBiolTher,2007.6:1676-83.
3.McDonald,E.R.,3rdandW.S.El-Deiry,Suppressionofcaspase-8-and-10-associatedRINGproteinsresultsinsensitizationtodeathligandsandinhibitionoftumorcellgrowth.ProcNatlAcadSciUSA,2004.101:6170-5.
4.Liao,W.,etal.,CARP-2isanendosome-associatedubiquitinligaseforRIPandregulatesTNF-inducedNF-kappaBactivation.CurrBiol,2008.18:641-9.
2.Yang,W.andW.S.El-Deiry,CARPsareE3ligasesthattargetapicalcaspasesandp53.CancerBiolTher,2007.6:1676-83.
3.McDonald,E.R.,3rdandW.S.El-Deiry,Suppressionofcaspase-8-and-10-associatedRINGproteinsresultsinsensitizationtodeathligandsandinhibitionoftumorcellgrowth.ProcNatlAcadSciUSA,2004.101:6170-5.
4.Liao,W.,etal.,CARP-2isanendosome-associatedubiquitinligaseforRIPandregulatesTNF-inducedNF-kappaBactivation.CurrBiol,2008.18:641-9.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Ubiquitination(UbiquitinLigase(E3))
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
