Bpsbioscience/PARP1 Chemiluminescent Assay Kit/80551/96 reactions
商品编号：
80551
品牌：
bpsbioscience
市场价：
¥10500.00
美元价：
6300.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
PARP1isknowntocatalyzetheNAD-dependentadditionofpoly(ADP-ribose)tohistones.
Description:
ThePARP1ChemiluminescentActivityAssaykitisdesignedtomeasurePARP1activityforscreeningandprofilingapplications.ThekeytothePARP1ChemiluminescentActivityAssayisthebiotinylatedsubstrate.Withthiskit,onlythreesimplestepsarerequiredforPARP1reactions.First,histoneproteinsarecoatedona96-wellplate.Next,thePARP1biotinylatedsubstrateisincubatedwiththeassaybufferthatcontainsthePARP1enzyme.Finally,theplateistreatedwithstreptavidin-HRPfollowedbyadditionoftheHRPsubstratetoproduceachemiluminescencethatcanthenbemeasuredusingachemiluminescencereader.
Synonym(s):
poly(ADP-ribose)polymerase1,poly(ADP-ribosyl)transferase,ADPRT,PARP1
SuppliedAs:
ThePARP1assaykitcomesinaconvenient96-wellformat,withpurifiedPARP1enzyme,histonemixture,activatedDNA,andPARP1assaybufferfor100enzymereactions.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:
COMPONENTS:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
Brown,J.A.,Marala,R.B.,J.Pharmacol.Toxicol.Methods.2002;47:137-41.
ApplicationReference(s):
1. Neuroprotectiveeffectsofanovelpoly(ADP-ribose)polymerase-1inhibitor,JPI-289,inhypoxicratcorticalneurons (2017)
2. DichamanetininhibitscancercellgrowthbyaffectingROS-relatedsignalingcomponentsthroughmitochondrial-mediatedapoptosis(2013)
3.StructuralbasisandSARforG007-LK,aleadstage1,2,4-triazolebasedspecifictankyrase1/2inhibitor(2013)
Notes:
Thiskitisalsosuitableforusewithcellextracts,butnonspecificribosylationactivitymaybedetected.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PARP
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
