Bpsbioscience/PDE10A1, GST-tag/60099/10 µg
商品编号：
60099
品牌：
bpsbioscience
市场价：
¥8800.00
美元价：
5280.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 116 kDa
Genbank #: NM_001130690
Tag(s): N-terminal GST-tag
a.a: 2–789(end)
Description:
Human PDE10A1 (GenBank Accession No. NM_001130690), a.a. 2-789(end) with N-terminal GST-tag, MW=116 kDa, expressed in Sf9 cells via a Baculovirus expression system.
UniProt
Q9Y233
Synonym(s):
PDE10A1, phosphodiesterase 10A1
Specific Activity:
≥ 3200 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme
that will convert 1 pmole of 3' , 5' -cAMP to
5' -AMP per minute at 37°C.
Assay Conditions:
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and 0.25-4 ng/µl PDE10A1 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
45 mM Tris-HCl, pH 8.0,
124 mM NaCl, 2.4 mM KCl, 20 mM
glutathione, 3 mM DTT and 10% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Gross-Langenhoff,M., et al., J. Biol. Chem. 281 (5), 2841-2846 (2006).
2. Frame,M., et al., Cell. Signal. 13 (10), 735- 741 (2001).
Product Specific Reference(s):
1. Demirbas, D., et al., PLoS One. 8(8), (2013).
2. Wang, Z., et al., J Sex Med. 10(11), 2790-7 (2013).
Application References:
1. Utility of Adenosine Monophosphate Detection System for Monitoring the Activities of Diverse Enzyme Reactions (2017)
2. Identification and characterization of a potent and biologically-active PDE4/7 inhibitor via fission yeast-based assays (2017)
3. Suppression of β-catenin/TCF transcriptional activity and colon tumor cell growth by dual inhibition of PDE5 and 10 (2015)
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
