Bpsbioscience/人脑源性神经营养因子/90110-B/10µg
商品编号：
90110-B
品牌：
bpsbioscience
市场价：
¥4100.00
美元价：
2460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:14kDa
Genbank#:P23560
a.a:129–247
Background:
BDNFisfoundinneuronsofthecentralnervoussystem.Itisexpressedpredominantlyinhippocampus,cortex,andsynapsesofthebasalforebrain.TheBIOLOGicalactivityofBDNFismediatedbyareceptorthatbelongstothetrkfamilyofreceptorsencodingatyrosine-specificproteinkinase.BDNFonlybindsweaklytothegp140trkreceptor(towhichNGFbindswithhighaffinity),anditbindstotheNGFreceptorknownasLNGFR.BDNFselectivelysupportsthesurvivalofprimarysensoryneuronsandretinalganglia.Thefactorsupportssurvivalanddifferentiationofcertaincholinergicneuronsandalsosomedopaminergicneuronsinvitro.BDNFdoesnotappeartoactonsympatheticganglia.InspecificneuronsofthecentralnervoussystemlocatedinthehippocampusandthecortexthesynthesisofBDNFisinfluencedbyneuronalactivityeitherpositively(glutamatetransmittersystem)ornegatively(GABAtransmittersystem).
Description:
RecombinantBDNFisadisulfide-linkedhomodimericproteinconsistingoftwo119aminoacidresidues.migratesasanapproximately27kDaproteinundernon-reducingconditionsandasa13-14kDaproteinunderreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingHumanBrainDerivedNeurotrophicFactormaturechainwasexpressedinE.coli.
UniProt
P23560
Synonym(s):
Brain-DerivedNeurotrophicFactor,BDNF,Abrineurin
Purity:
≥97%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BiologicalActivity:
TheED50wasdeterminedbythedose-dependentproliferationof ratC6cells,andwasfoundtobeintherangeof 0.5µg/ml.
Formulation:
Lyophilizedfrom0.2µmfilteredcitricacid,pH5.7.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.PNAS,Sep2009,106:16481-16486.
2.J.Neurosci.,Sep2009,29:11650-11661.
2.J.Neurosci.,Sep2009,29:11650-11661.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
HSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
