Bpsbioscience/组蛋白H3(K9)通用甲基转移酶新检测试剂盒/52072/96反应
商品编号:
52072
品牌:
bpsbioscience
市场价:
¥16000.00
美元价:
9600.00
产品分类:
其它检测试剂盒
公司分类:
Other_kits
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Background:
HistoneH3(K9)methyltransferasesareenzymesthatcatalyzethetransferofamethylgroupfromthecofactorS-adenosylmethioninetolysine9residueofhistoneH3.ThisgroupofenzymesincludesG9a,SUV39H1,andSUV39H2.
Description:
TheHistoneH3(K9)UniversalMethyltransferaseAssayKitisdesignedforthedetectionofhistoneH3(K9)methyltransferase(HMT)activityinscreeningandprofilingapplications.ThekeytotheHistoneH3(K9)UniversalMethyltransferaseAssayKitisahighlyspecificantibodythatrecognizesmethylatedK9residueofHistoneH3.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequiredformethyltransferasedetection.First,Sadenosylmethionineisincubatedwithasamplecontainingassaybufferandmethyltransferaseenzymeforonehour.Next,primaryantibodyisadded.Finally,theplatesaretreatedwithanHRP-labeledsecondaryantibodyfollowedbyadditionoftheHRPsubstratetoproducechemiluminescencethatcanthenbemeasuredusingachemiluminescencereader.
SuppliedAs:
Kitcomesinaconvenientformat,witha96-wellplateprecoatedwithhistoneH3peptidesubstrate,purifiedG9a,SUV39H1andSUV39H2,andreagentsfor100enzymereactions.
Format:
COMPONENTS:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
DillonS.C.,etal.2005.GenomeBIOLOGy6:227.
ApplicationReference(s):
DNAmethyltransferase3aregulatesosteoclastdifferentiationbycouplingtoanS-adenosylmethionine-producingmetabolicpathway(2015)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Methyltransferase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。