Bpsbioscience/EZH1 Chemiluminescent Assay Kit/52990/384 reactions
商品编号：
52990
品牌：
bpsbioscience
市场价：
¥82400.00
美元价：
49440.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Catalog#:52990
Size:384reactions
Contactusforpricing
Species:Human
Description:
TheEZH1ChemiluminescentAssayKitisdesignedtomeasureactivityoftheEZH1complex(EZH1/EED/SUZ12/RbAp48/AEBP)forscreeningandprofilingpurposes.ThekeytotheEZH1AssayKitisahighlyspecificantibodythatrecognizesmethylatedHistoneH3K27.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequiredformethyltransferasedetection.First,S-adenosylmethionineisincubatedwithasamplecontainingassaybufferandmethyltransferaseenzymefortwohours.Next,primaryantibodyisadded.Finally,theplateistreatedwithanHRP-labeledsecondaryantibodyfollowedbyadditionoftheHRPsubstratetoproducechemiluminescencethatcanthenbemeasuredusingachemiluminescencereader.
MaterialsRequiredButNotProvided:
TBSTbuffer(1xTris-bufferedsaline,pH8.0,containing0.05%Tween-20)
LuminometerorfluorescentmicroplatereadercapableofreADIngchemiluminescence
Rotatingorrockerplatform
UniProt
Q92800
Synonym(s):
KMT6B,EZH
SuppliedAs:
TheEZH1ChemiluminescentAssayKitcomesinaconvenientformat,withwellsprecoatedwithhistoneH3peptidesubstrate,anantibodyagainstmethylatedK27residueofHistoneH3,thesecondaryHRP-labeledantibody,S-adenosylmethionine,methyltransferase
Format:
Instructionsforuse:
Seeproductdatasheetfordetailedprotocol.
Storage/StABIlity:
Oneyearfromdateofreceiptwhenstoredasdirected.
Application(s):
GreatforstudyingenzymekineticsandHTSapplications.
Reference(s):
1.Dillon,S.C.,etal. GenomeBIOLOGy2005;6:227.
2.Morin,R.D.,etal.NatGenet.2010;42(2):181.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Methyltransferase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
