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Bpsbioscience/EHMT1/GLP Chemiluminescent Assay Kit/53007/96 reactions

Bpsbioscience/EHMT1/GLP Chemiluminescent Assay Kit/53007/96 reactions
商品编号:
53007
品牌:
bpsbioscience
市场价:
¥14300.00
美元价:
8580.00
产品分类:
其它检测试剂盒
公司分类:
Other_kits
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Background:
EHMT1(GLP)isahistonemethyltransferasethatexhibitsmethylationactivitytowardH3-K9.
Description:
TheEHMT1(GLP)DirectActivityChemiluminescentAssayKitisdesignedtomeasureEHMT1,alsoknownasG9a-likeproteinorGLPactivityforscreeningandprofilingapplications.ThekeytotheEHMT1(GLP)ChemiluminescentAssayKitisahighlyspecificantibodythatrecognizesmethylatedK9residueofH3.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequiredformethyltransferasedetection.First,S-adenosylmethionineisincubatedwithasamplecontainingassaybufferandmethyltransferaseenzymeforonehour.Next,primaryantibodyisadded.Finally,theplateistreatedwithanHRP-labeledsecondaryantibodyfollowedbyadditionoftheHRPsubstratetoproducechemiluminescencethatcanthenbemeasuredusingachemiluminescencereader.
Synonym(s):
EHMT1,GLP,KMT1D,G9a-likeprotein,euchromatichistone-lysineN-methyltransferase
SuppliedAs:
TheEHMT1(GLP)ChemiluminescentAssayKitcomesinaconvenientformat,witha96-wellplateprecoatedwithhistoneH3peptidesubstrate,reagents,andpurifiedEHMT1for100enzymereactions.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
Huang,J.,etal.,JBiol.Chem.2010Mar26;285(13):9636-41.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Methyltransferase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。