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Bpsbioscience/DOT1L (KMT4) Chemiluminescent Assay Kit/52202/96 reactions
Bpsbioscience/DOT1L (KMT4) Chemiluminescent Assay Kit/52202/96 reactions
商品编号：
52202
品牌：
bpsbioscience
市场价：
¥14300.00
美元价：
8580.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:
TheDOT1LChemiluminescentAssayKitisdesignedtomeasureDOT1Lactivityforscreeningandprofilingapplications.ThekeytotheDOT1LChemiluminescentAssayKitisahighlyspecificantibodythatrecognizesmethylatedK79residueofH3.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequiredformethyltransferasedetection.First,S-adenosylmethionineisincubatedwithasamplecontainingassaybufferandmethyltransferaseenzymefortwohours.Next,primaryantibodyisadded.Finally,thestripsaretreatedwithanHRP-labeledsecondaryantibodyfollowedbyadditionofHRPsubstratetoproducechemiluminescencethatcanthenbemeasuredusingachemiluminescencereader.
Synonym(s):
KMT4,KIAA1814
SuppliedAs:
TheDOT1LChemiluminescentAssayKitcomesinaconvenientformat,witha96-wellplate,DOT1Lsubstrate,primaryantibodyagainstmethylatedlysineresidueofHistoneH3,secondaryHRP-labeledantibody,Sadenosylmethionine,methyltransferaseassaybuffer
Format:
COMPONENTS:
Instructionsforuse:
Seeproductdatasheetfordetailedprotocol.
Storage/StABIlity:
Oneyearfromdateofreceiptwhenstoredasdirected.
Application(s):
GreatforstudyingenzymekineticsandHTSapplications.
Reference(s):
1.Min,J.,etal.Cell2003;112:711-723.
2.Daigle,S.R.,etal.CancerCell2011,20:53-65.
2.Daigle,S.R.,etal.CancerCell2011,20:53-65.
Notes:
MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
TBSTbuffer(1xTris-bufferedsaline,pH8.0,containing0.05%Tween-20)
LuminometerorfluorescentmicroplatereadercapableofreADIngchemiluminescence
Rotatingorrockerplatform
TBSTbuffer(1xTris-bufferedsaline,pH8.0,containing0.05%Tween-20)
LuminometerorfluorescentmicroplatereadercapableofreADIngchemiluminescence
Rotatingorrockerplatform
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Methyltransferase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
