Bpsbioscience/Carbonic Anhydrase 9 (CA9), His-tag/71101/100 µg
商品编号：
71101
品牌：
bpsbioscience
市场价：
¥9600.00
美元价：
5760.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:39.7kDa
Genbank#:NM_001216
Tag(s):C-terminalHis-tag
a.a:59–414
Background:
CAIXisatransmembraneproteinandtheonlytumor-associatedcarbonicanhydraseisoenzymeknown.Itisexpressedinallclear-cellrenalcellcarcinoma,butisnotdetectedinnormalkidneyormostothernormaltissues.Expressionofthemembrane-boundcarbonicanhydrase,CAIX,hasbeenreportedinhypoxictumorswhereitsactivityislinkedtopromotingsurvivaloftumorcellsandaidingcancerprogression.Thisisthoughttooccurthroughthecarbonicanhyrase-mediatedproductionofbicarbonateions,whichneutralizestheabnormallyacidicpHoftheextracellularenvironmentinducedduringhypoxia.FurThermore,asCAIXisahypoxia-andHIF-1-inducIBLeenzyme,itsexpressioncanbeusedasabioMarkerforhypoxictumors.
Description:
HumancarbonicanhydraseIX(CAIX),alsoknownasCA9,(GenBankAccessionNo.NM_001216),a.a.59-414withC-terminalHis-tag,MW=39.7kDa,expressedinabaculovirusinfectedSf9cellexpressionsystem.
UniProt
Q16790
Synonym(s):
CarbonicAnhydraseIX,PMW,RCC-AssociatedAntigenG250,CAIX,CarbonicAnhydrase9,CarbonateDehydratase,CA9
SpecificActivity:
≥30,000pmol/min/µg
AssayConditions:
15mMTris-HCl,pH7.4,100mMNaCl,and3mMp-Nitrophenylacetate(PNPA).Incubatefor30minutesatroomtemperature.Absorbanceismeasuredat405nM.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,266mMimidazoleand20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Hilvo,M.,etal.,JBiolChem.2008Oct10;283(41):27799-809.
2.Chadwick,A.L.,etal.,CellCycle.2013Jul30;12(16):2534.
2.Chadwick,A.L.,etal.,CellCycle.2013Jul30;12(16):2534.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
MetabolicEnzymes-Transferase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
