Bpsbioscience/ATP柠檬酸裂解酶，GST标签/50255/10µg
商品编号：
50255
品牌：
bpsbioscience
市场价：
¥9800.00
美元价：
5880.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 147 kDa
Genbank #: NM_001096
Tag(s): N-terminal GST-tag
a.a: full length
Background:
ATP citrate-lyase is an important enzyme for cytosolic acetyl-CoA synthesis. During fatty acid synthesis, Citrate and CoA form acetyl-CoA and oxaloacetate.
ATP citrate-lyase has emerged as new target for cancer treatment. Studies have found that many types of cancer including glioblastoma, colorectal cancer, breast cancer, non-small cell lung cancer, and hepatocellular carcinoma experience abnormally high levels of ATP citrate-lyase.
Description:
Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST-tag, MW=147 kDa, expressed in baculovirus expression system.
UniProt
P53396
Synonym(s):
ATP citrate lyase, ACLY
Specific Activity:
≥300 pmole/min/μg.
Assay Conditions:
A 25 µl ACLY reaction is conducted in a buffer
containing 40 mM Tris (pH 8.0), 10 mM MgCl2,
2 mM DTT, 2 mM potassium citrate, 0.1 mM CoA
and 6-100 ng human ACLY at 30 oC for 60 min.
Enzyme activity was assayed using ADP-Glo
reagents (Promega)
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Migita T. et al., Cancer Res. 68 (20), 8547- 8554 (2008).
2. Ramakrishna S. et al., Biochemistry 29 (33), 7617-7624 (1990).
Application Reference:
ATP citrate lyase mediates resistance of colorectal cancer cells to SN38
(2013)
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes-Lyase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
