Bpsbioscience/ALOX15,标记/50220/20µg
商品编号:
50220
品牌:
bpsbioscience
市场价:
¥9400.00
美元价:
5640.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:75kDa
Genbank#:NM_001140
Tag(s):N-terminalFLAG-tag
a.a:2–662(end)
Description:
ALOX-15,alsoknownas15LOX-1,omega-6lipoxygenase,orarachidonate15-lipoxygenase(GenBankAccessionNo.NM_001140),a.a.2-662(end),withN-terminalFLAG-tag,MW=75kDa,expressedinSf9insectcellsviaabaculovirusexpressionsystem.
UniProt
P16050
Synonym(s):
15LOX-1,omega-6lipoxygenase,arachidonate15-lipoxygenase
AssayConditions:
ALOXactivitywasmeasuredusingaDPPP(diphenyl-1-Pyrenylphosphine)coupledassay.Theenzyme(5.6ng/µl)wasincubatedwith200µMlinoleicacidinDPBSbuffer(pH7.4)for10minatroomtemperaturefollowedbyadditionof100µlof0.125mMDPPPinmethanol.After30minincubationatroomtemperature,fluorescencewasreadatEx350nm/Em380nm.
Formulation:
50mMTris-HCl,pH8.0,138mMNaCl,2.7mMKCl,and100ng/µlFLAGpeptide.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Jiang,W.G.,etal.(2006).ProstaglandinsLeukotEssentFattyAcids.74(4):235-45.
2.Sigal,E.,etal.(1988).Biochem.Biophys.Res.Commun.157(2):457-64.
3.BhattacharyaS,etal.(2009).TumourBiol.30(4):185-99.
2.Sigal,E.,etal.(1988).Biochem.Biophys.Res.Commun.157(2):457-64.
3.BhattacharyaS,etal.(2009).TumourBiol.30(4):185-99.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
MetabolicEnzymes-Oxygenase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。