Bpsbioscience/HSP90β(C端)抑制剂筛选试剂盒/50314/384反应
商品编号:
50314
品牌:
bpsbioscience
市场价:
¥19500.00
美元价:
11700.00
产品分类:
其它检测试剂盒
公司分类:
Other_kits
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Description:
TheHSP90β(C-terminal)InhibitorScreeningAssayKitisdesignedtomeasuretheinhibitionofHSP90βbindingtoitsproteintargetPPID(alsoknownasCyclophilinD).Withthiskit,onlythreesimplestepsonamicrotiterplatearerequired.First,asamplecontainingHSP90β,PPID,andaninhibitorofchoiceisincubatedforthirtyminutes.Next,acceptorbeadsareadded,thendonorbeads,followedbyreADIngtheAlpha-counts.
UniProt
P08238
Synonym(s):
Hsp90beta,heatshockprotein90beta
SuppliedAs:
KitcomesinaconvenientAlphaLISA®format,withenoughHSP90β(527-724),assaybuffer,detectionbuffer,andpurifiedGST-taggedPPIDtoperformatotalof384enzymereactions.
Contraindications:
AvoidDMSOconcentrationsabove0.5%.AvoidgreenandbluedyesthatabsorblightintheAlphascreen®signalemissionrange(520-620nm),suchasTrypanBlue.Avoidtheuseofthepotentsingletoxygenquencherssuchassodiumazide(NaN3)ormetalions(Fe2+,Fe3+,Cu2+,Zn2+andNi2+).Thepresenceof>1%RPMI1640culturemediumleadstoasignalreductionduetothepresenceofexcessbiotinandironinthismedium.MEM,whichlacksthesecomponents,doesnotaffectAlphascreen®assays.
Format:
COMPONENTS:
Storage/StABIlity:
Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
UsefulforscreeninginhibitorsofHSP90βandforHSP90βbindingassays
Reference(s):
Allan,R.K.etal.J.Biol.Chem2006281(11):7161-71.
Notes:
AlphaScreen®andAlphaLISA®areregisteredtrademarksofPerkinelmer,Inc.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
HeatShockProteins
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。