Bpsbioscience/HSP90α (N-terminal) Assay Kit/50298/384 reactions
商品编号：
50298
品牌：
bpsbioscience
市场价：
¥19500.00
美元价：
11700.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
Hsp90αisamolecularchaperonewithessentialfunctionsinmaintainingtransformation.InhibitionofHsp90α functionhasbeenshowntoplayaroleintumOrigenesisanddiseaseprogression.
Description:
TheHSP90α(N-terminal)AssayKitisdesignedforidentificationofHSP90αinhibitorsusingfluorescencepolarization.TheassayisbasedonthecompetitionoffluorescentlylabeledgeldanamycinforbindingtopurifiedrecombinantHSP90α.ThekeytotheHSP90αAssayKitisthefluorescentlylabeledgeldanamycin.Usingthiskit,onlyonesimplesteponamicrotiterplateisrequiredforHSP90αreactions.ThefluorescentlylabeledgeldanamycinisincubatedwithasamplecontainingHSP90αenzymetoproduceachangeinfluorescentpolarizationthatcanthenbemeasuredusingafluorescencereader.
UniProt
P07900
Synonym(s):
HSP90a,HeatShockProtein90a,HSP90alpha,HSP90α
SuppliedAs:
TheHSP90αinhibitorscreeningassaykitcomesinaconvenient384-wellformat,withpurifiedHSP90αenzyme,fluorescentlylabeledgeldanamycinandHSP90assaybufferfor384enzymereactions.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:
COMPONENTS:
Instructionsforuse:
Seedatasheetfordetailedprotocol.
Storage/StABIlity:
Stableforatleast12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
1.Kim,J.,etal.,JBiomolScreening.2004,9(5):375-381.
2.Howes,R.,etal.,AnalBiochem.2006,350:202-213.
ApplicationReference(s):
1. RationalDesignofSelectiveAdenine-BasedScaffoldsforInactivationofBacterialHistidineKinases(2017)
ProductSpecificReference(s):
1.Shaaban,KA.,etal., JNatProd. 2013,76(9):1619-26.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
HeatShockProteins
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
