Bpsbioscience/HSP90α（C端）抑制剂筛选分析试剂盒/50317/384反应
商品编号：
50317
品牌：
bpsbioscience
市场价：
¥19500.00
美元价：
11700.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:
TheHSP90α(C-Terminal)InhibitorScreeningAssayKitisdesignedtomeasuretheinhibitionofHSP90αbindingtoitsproteintargetPPID(alsoknownasCyclophilinD).Withthiskit,onlythreesimplestepsonamicrotiterplatearerequired.First,asamplecontainingHSP90α,PPID,andaninhibitorofchoiceisincubatedforthirtyminutes.Next,acceptorbeadsareadded,thendonorbeads,followedbyreADIngtheAlpha-counts.
UniProt
P07900
Synonym(s):
HSP90alpha,HeatShockProtein90alpha
SuppliedAs:
TheassaykitcomesinaconvenientAlphaLISA®format,withenoughHSP90α(535-732),assaybuffer,detectionbuffer,andpurifiedGST-taggedPPIDtoperformatotalof384enzymereactions.
Contraindications:
OnlylimitedamountsofDMSO(<1%)canbeincludedasithasbeenshowntodisruptHSP90α:PPIDinteraction.GreenandbluedyesthatabsorblightintheAlphaScreen®signalemissionrange(520-620nm),suchasTrypanBlue.Avoidtheuseofthepotentsingletoxygenquencherssuchassodiumazide(NaN3)ormetalions(Fe2+,Fe3+,Cu2+,Zn2+andNi2+).Thepresenceof>1%RPMI1640culturemediumleadstoasignalreductionduetothepresenceofexcessbiotinandironinthismedium.MEM,whichlacksthesecomponents,doesnotaffectAlphaScreen®assays.
Format:
Storage/StABIlity:
Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
UsefulforscreeninginhibitorsofHSP90αandforHSP90αbindingassays
Reference(s):
Allan,R.K.etal.J.Biol.Chem2006. 281(11):7161-71.
Notes:
AlphaScreen® andAlphaLISA® areregisteredtrademarksofPerkinelmer,Inc.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
HeatShockProteins
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
