Bpsbioscience/FBXL10 (KDM2B, JHDM1B), FLAG-tag/50120/20 µg
商品编号：
50120
品牌：
bpsbioscience
市场价：
¥9500.00
美元价：
5700.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:75kDa
Genbank#:NM_032590
Tag(s):C-terminalFLAG-tag
a.a:1–650
Description:
HumanF-BoxProtein10(FBXL10,alsoknownasKDM2B,JHDM1B,CXXC2,andHistone-H3K36demethylase1B),GenBankAccessionNo.NM_032590,a.a.1-650,withC-terminalFLAG-tag,MW=75kDa,expressedinSf9cellsviaabaculovirusexpressionsystem.
UniProt
Q8NHM5
Synonym(s):
F-BoxProtein10,FBXL10,KDM2B,JHDM1B,CXXC2,Histone-H3K36demethylase1B
Purity:
≥83%byHPLC
AssayConditions:
Enzymewasincubatedwith0.4μMsubstrateinHEPES-containingJMJassaybufferSAandSA-(withoutKG)ina10-μlreactionfor180minat30˚C.5μlofAnti-RabbitAlphaLISA®acceptorbeads(1:250)and5μlofrabbitpolyclonalantibody(1:200)wereaddedandincubationcontinuedfor30min.StreptavidinDonorbeads(1:125)wereadded(10μl)andmixturewasfurtherincubatedfor10minatRTbeforeAcountsweremeasured.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and80µg/mlFLAGpeptide
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Tanaka,Y.,etal.(2010).EMBOJ.29(9):1510-22.
2.Blackledge,N.P.,etal.(2010).Mol.Cell.38(2):179-90.3.Frescas,D.,etal.(2008).CellCycle7(22):3539-47.
2.Blackledge,N.P.,etal.(2010).Mol.Cell.38(2):179-90.3.Frescas,D.,etal.(2008).CellCycle7(22):3539-47.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Demethylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
