Bpsbioscience/LSD1（KDM1A）荧光分析试剂盒/50107/384反应
商品编号：
50107
品牌：
bpsbioscience
市场价：
¥12100.00
美元价：
7260.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
LSD1isachromatin-modifyingenzymethatspecificallyremovesmethylgroupsfrommono-anddimethylatedLysofhistoneH3.LSD1isacriticalcomponentoftranscriptionalregulationviaepigenetichistonemodificationsandisthereforeapotentialtargetfordrugdevelopment.
Description:
TheLSD1AssayKitisdesignedtomeasureLSD1activityofpurifiedLSD1enzymescontainingLSD1usingfluorimetry.TheassayisbasedontheanalysisofthehydrogenperoxideproducedduringthedemethylationreactionmediatedbyLSD1.ThekeytotheLSD1AssayKitisthemethylatedpeptidesubstrate.Usingthiskit,onlytwosimplestepsonamicrotiterplatearerequiredforLSD1reactions.First,asamplecontainingLSD1isincubatedinareactionmixturewiththemethylatedpeptidesubstrate.Second,peroxidaseandAmplexRedreagentareaddedandfluorescence(λex=530±13nm,λem=590±18nm)ismeasuredusingaplatereader.
UniProt
O60341
Synonym(s):
KDM1A,AOF2,LSD1,BHC110,LSD
SuppliedAs:
TheLSD1AssayKitisprovidedinaconvenient384-wellformat,withpurifiedLSD1,AmplexRedreagent,peroxidase,andLSD1assaybufferfor384enzymereactions.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
1.FornerisF,etal.JBiolChem2006;281(46):35289-95.
2.ZhouM,etal.AnalBiochem1997;253(2):162-8.
2.ZhouM,etal.AnalBiochem1997;253(2):162-8.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Demethylase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
