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当前位置: 首页 > 产品中心 > Other_kits > Bpsbioscience/SIRT1(Sirtuin1)荧光检测试剂盒/50081/96反应
商品详细Bpsbioscience/SIRT1(Sirtuin1)荧光检测试剂盒/50081/96反应
Bpsbioscience/SIRT1(Sirtuin1)荧光检测试剂盒/50081/96反应
Bpsbioscience/SIRT1(Sirtuin1)荧光检测试剂盒/50081/96反应
商品编号: 50081
品牌: bpsbioscience
市场价: ¥8900.00
美元价: 5340.00
产地: 美国(厂家直采)
公司:
产品分类: 其它检测试剂盒
公司分类: Other_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description:
TheFluorogenicSIRT1AssayKitisacompleteassaysystemdesignedtomeasureSirtuin1(SIRT1)activityforscreeningandprofilingapplications.TheFluorogenicSIRT1AssayKitisbasedonauniquefluorogenicsubstrateanddevelopercombination.ThisassaymethodeliminatesdealingwiththerADIoactivity,extraction,andchromatographyaspectsoftraditionalassays.Usingthiskit,onlytwosimplestepsonamicrotiterplateareneededtoanalyzetheSIRT1activitylevel.First,theSIRTfluorometricsubstrate(SIRTsubstrate1),containinganacetylatedlysinesidechain,isincubatedwithpurifiedSIRT1enzyme.ThedeacetylationsensitizesthesubstratesosubsequenttreatmentwiththeLysineDeveloperproducesafluorophorethatcanthenbemeasuredusingafluorescencereader.
UniProt
Q96EB6
Synonym(s):
sirt1,Sir2,Sir2a
SuppliedAs:
Kitcomesinaconvenient96-wellformat,withreagents,purifiedSIRT1enzymeandaSIRTinhibitor,Nicotinamide,foruseasapositiveandnegativecontrolnecessaryfor100fluorescentSIRT1(Sir2)activitymeasurements.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:

COMPONENTS:

Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:

12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.

Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):

1.A.Itoetal.(2001)EMBOJ.201331.
2.A.Itoetal.(2002)EMBOJ.216236.
3.N.A.Barlevetal.(2001)Mol.Cell81243.

ApplicationReference(s):
Middle-agedratsorallysupplementedwithgel-encapsulatedcatechinfavorablyincreasesbloodcytosolicNADPHlevels(2015)

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Deacetylase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。