Bpsbioscience/HDAC2荧光检测试剂盒/50062/96反应
商品编号：
50062
品牌：
bpsbioscience
市场价：
¥8900.00
美元价：
5340.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
HDACsregulatecellularprocessesbycatalyzingthehydrolysisofanacetylgroupfromacetyllysinesinmodifiedproteins.IntheHDACassay,fluorescent-dyemoleculesareattachedtoapeptidecontainingacetyllysine.Attachmenttothepeptidequenchesthefluorescenceofthedye.AftertreatmentofthepeptidewithanHDAC,thereactionismixedwithadevelopmentsolutionthatisspecificfornonacetylatedlysines.IftheacetylgrouphasbeenremovedfromthelysinebytheHDAC,thissolutionwillreleasethedyeallowingforfluorescence.FluorescenceisthereforedirectlyrelatedtoHDACactivity.
Description:
TheFluorogenicHDAC2AssayKitisacompleteassaysystemdesignedtomeasurehistonedeacetylase2(HDAC2)activityforscreeningandprofilingapplications.Itcomesinaconvenient96-wellformat,withallthereagentsnecessaryfor100fluorescentHDAC2activitymeasurements.Inaddition,thekitincludespurifiedHDAC2enzymeandapotentHDACinhibitor,TrichostatinA,foruseasapositiveandnegativecontrol.TheFluorogenicHDAC2AssayKitisbasedonauniquefluorogenicsubstrateanddevelopercombination.ThisassaymethodeliminatesdealingwiththerADIoactivity,extraction,andchromatographyaspectsoftraditionalassays.Usingthiskit,onlytwosimplestepsonamicrotiterplateareneededtoanalyzetheHDACactivitylevel.First,theHDACfluorometricsubstrate,containinganacetylatedlysinesidechain,isincubatedwithpurifiedHDAC2.ThedeacetylationsensitizesthesubstratesosubsequenttreatmentwiththeLysineDeveloperproducesafluorophorethatcanthenbemeasuredusingafluorescencereader.
UniProt
Q92769
Synonym(s):
histonedeacteylase,HDAC2
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:
COMPONENTS:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsofHDAC2fordrugdiscoveryandHTSapplications.
Reference(s):
1.Santo,L.,etal.,Blood.2012Mar15;119(11):2579-89.
2.Bradner,J.E.,etal.,NatChemBiol.2010Mar;6(3):238-243.
ApplicationReferences:
1. Design,synthesis,andBIOLOGicalactivityofNCC149derivativesashistonedeacetylase8-selectiveinhibitors(2014)
2.Preclinicalanti-arthriticstudyandpharmacokineticpropertiesofapotenthistonedeacetylaseinhibitorMPT0G009(2014)
3. PromiscuousactionsofsmallmoleculeinhibitorsoftheproteinkinaseD-classIIaHDACaxisinstriatedmuscle(2015)
4. Explorationofnovel5′(7′)-substituted-2′-oxospiro[1,3]dioxolane-2,3′-indoline-basedN-hydroxypropenamidesashistonedeacetylaseinhibitorsandantitumoragents
Notes:
MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
0.1%solution(1mg/ml)ofbovineserumalbumin(BSA)inwater
Fluorimetercapableofexcitationat350-380nmanddetectionat440-460nm
Adjustablemicropipettorandsteriletips
Rotatingorrockerplatform
0.1%solution(1mg/ml)ofbovineserumalbumin(BSA)inwater
Fluorimetercapableofexcitationat350-380nmanddetectionat440-460nm
Adjustablemicropipettorandsteriletips
Rotatingorrockerplatform
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Deacetylase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
