Bpsbioscience/人酸性成纤维细胞生长因子/90101-A/10µg
商品编号：
90101-A
品牌：
bpsbioscience
市场价：
¥2600.00
美元价：
1560.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:16kDa
Genbank#:P05230
a.a:16–155
Background:
aFGFandbFGFdisplay55%homologywhichislimited,however,toafewfunctionaldomains.ThestructureofaFGF,revealedbyX-raycrystallography,showsasimilarfoldingstructureasIL-1.BecauseaFGFbindstothesamereceptorasbFGF,aFGFdisplaysmoreorlessthesamespectrumofactivities.Itis,however,generallylessactivethanbFGFinsimilarassays.FGFreceptorsareencodedbyagenefamilyconsistingofatleastfourreceptortyrosinekinasesthattransducesignalsimportantinavarietyofdevelopmentalandphysiologicalprocessesrelatedtocellgrowthanddifferentiation.
Description:
RecombinanthumanaFGF(acidicFibroblastGrowthFactor)isadisulfide-linkedmonomerproteinconsistingof141a.a.andmigratesasanapproximately16kDaproteinunderreducingandnon-reducingconditions.NativehumanaFGF,generatedbytheproteolyticremovalofthesignalpeptideandpropeptide,hasacalculatedmassof16kDa.OptimizedDNAsequenceencodinghumanacidicFibroblastGrowthFactormaturechainwasexpressedinE.coli.
UniProt
P05230
Synonym(s):
aFGF,acidicFibroblastGrowthFactor,Heparin-BindingGrowthFactor1,HBGF-1,FGF-1,FibroblastGrowthFactor-1
Purity:
≥98%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BIOLOGicalActivity:
TheED50asdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingFGFreceptorsis<10ng/ml,correspondingtoaspecificactivityof≥1x105units/mg.
Formulation:
Lyophilizedfrom0.2µmfilteredsolutioncontaining2.5%glycine,0.5%sucrose,0.01%Tween-80,5mMglutamicacid,pH4.5.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.Expressionofacidicfibroblastgrowthfactor(aFGF)andfibroblastgrowthfactorreceptor4(fgfr4)inbreastfibroadenomas.J.Clin.Pathol.,Jan2001,54:37-41.
2.Requirementofphosphatidylinositol3-kinaseactivityfortranslocationofexogenousaFGFtothecytosolandnucleus.J.Biol.Chem.,Apr2000,275:11972.
2.Requirementofphosphatidylinositol3-kinaseactivityfortranslocationofexogenousaFGFtothecytosolandnucleus.J.Biol.Chem.,Apr2000,275:11972.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
FNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
