Bpsbioscience/TCF/LEF报告试剂盒（Wnt信号通路）/60500/500反应
商品编号：
60500
品牌：
bpsbioscience
市场价：
¥11300.00
美元价：
6780.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
TheWnt/β-cateninsignalingpathwaycontrolsalargeanddiversesetofcellfatedecisionsinembryonicdevelopment,adultorganmaintenanceanddisease.Wntproteinsbindtoreceptorsonthecellsurface,initiatingasignalingcascadethatleadstostABIlizationandnucleartranslocationofβ-catenin.β-cateninthenbindstoTCF/LEFtranscriptionfactorsinthenucleus,leADIngtotranscriptionandexpressionofWnt-responsivegenes.
Description:
TheTCF/LEFReporterkitisdesignedformonitoringtheactivityofWnt/β-cateninsignalingpathwayintheculturedcells.Thekitcontainstransfection-readyTCF/LEFluciferasereportervector,whichisaWntpathway-responsivereporter.ThisreportercontainsafireflyluciferasegeneunderthecontrolofmultimerizedTCF/LEFresponsiveelementlocatedupstreamofaminimalpromoter.TheTCF/LEFreporterispremixedwithconstitutively-expressingRenillaluciferasevectorthatservesasinternalcontrolfortransfectionefficiency.Thekitalsoincludesanon-inducIBLefireflyluciferasevectorpremixedwithconstitutively-expressingRenillaluciferasevectorasnegativecontrol.Thenon-inducibleluciferasevectorcontainsafireflyluciferasegeneunderthecontrolofaminimalpromoter,withoutanyadditionalresponseelements.Thenegativecontroliscriticaltodeterminingpathwayspecificeffectsandbackgroundluciferaseactivity.
Synonym(s):
WNTreporter,TCFreporter,LEFreporter,luciferasereporter
SuppliedAs:
Reporter:TCF/LEFluciferasereportervector+constitutively-expressingRenillaluciferasevector
NegativeControlReporter:Non-inducibleluciferasevector+constitutively-expressingRenillaluciferasevector
NegativeControlReporter:Non-inducibleluciferasevector+constitutively-expressingRenillaluciferasevector
Concentration:
BoththeReporterandNegativeControlReporterareprovidedat60ngDNA/µl(500µleach)
Format:
assaykit
Instructionsforuse:
Seedatasheetfordetailedtransfectionandassayprotocols.
Storage/Stability:
Stableatleast12monthsfromdateofreceipt,whenstoredasdirected(-20°C)
Application(s):
•MonitorWnt/β-cateninsignalingpathwayactivity.
•ScreenforactivatorsorinhibitorsoftheWnt/β-cateninsignalingpathway.
•StudyeffectsofRNAiorgeneoverexpressionontheactivityoftheWnt/β-cateninpathway.
•ScreenforactivatorsorinhibitorsoftheWnt/β-cateninsignalingpathway.
•StudyeffectsofRNAiorgeneoverexpressionontheactivityoftheWnt/β-cateninpathway.
Reference(s):
1.CleversH(2006)Wnt/beta-cateninsignalingindevelopmentanddisease.Cell127(3):469-480.
2.ChenBetal.(2009)Smallmolecule-mediateddisruptionofWnt-dependentsignalingintissueregenerationandcancer.NatureChemicalBIOLOGy5(2):100-107.
2.ChenBetal.(2009)Smallmolecule-mediateddisruptionofWnt-dependentsignalingintissueregenerationandcancer.NatureChemicalBIOLOGy5(2):100-107.
Notes:
Thevectorsaresuitablefortransienttransfection.TheyareNOTsuitablefortransformationandamplificationinbacteria.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
CellSignalingPathway,WNT
ProductType:
ReporterAssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
