Bpsbioscience/SRE报告试剂盒（MAPK/ERK信号通路）/60511/500反应
商品编号：
60511
品牌：
bpsbioscience
市场价：
¥10500.00
美元价：
6300.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
TheMAPK/ERKsignalingpathwayisamajorparticipantintheregulationofcellgrowthanddifferentiation.Itcanbeactivatedbyvariousextracellularstimuliincludingmitogens,growthfactors,andcytokines.Uponstimulation,MEK1/2phosphorylateandactivateERK1/2.TheactivatedERKtranslocatestothenucleuswhereitphosphorylatesandactivatestranscriptionfactors.TheTCFs(TernaryComplexFactors),includingElk1,areamongthebest-characterizedtranscriptionfactorsubstratesofERK.WhenphosphorylatedbyERK,Elk1formsacomplexwithSerumResponseFactor(SRF)andbindstoSerumResponseElement(SRE),resultingintheexpressionofnumerousmitogen-inducIBLegenes.
Description:
TheSREReporterKitisdesignedformonitoringtheactivityoftheERKsignalingpathwayandthetranscriptionalactivityofSRFinculturedcells.Thekitcontainsatransfection-readySREluciferasereportervector,whichisanERKpathway-responsivereporter.ThisreportercontainsthefireflyluciferasegeneunderthecontrolofmultimerizedSREresponsiveelementslocatedupstreamofaminimalpromoter.TheSREreporterispremixedwithaconstitutively-expressingRenilla(seapansy)luciferasevectorthatservesasaninternalcontrolfortransfectionefficiency.Thekitalsoincludesanon-induciblefireflyluciferasevectorpremixedwithconstitutively-expressingRenillaluciferasevectorasanegativecontrol.Thenon-inducibleluciferasevectorcontainsthefireflyluciferasegeneunderthecontrolofaminimalpromoter,withoutanyadditionalresponseelements.Thenegativecontroliscriticalfordeterminingpathway-specificeffectsandthebackgroundluciferaseactivity.
Synonym(s):
SREreporter,MAPKreporter,ERKreporter
SuppliedAs:
Reporter:SREluciferasereportervector+constitutively-expressingRenillaluciferasevector
NegativeControlReporter:Non-inducibleluciferasevector+constitutively-expressingRenillaluciferasevector
NegativeControlReporter:Non-inducibleluciferasevector+constitutively-expressingRenillaluciferasevector
Concentration:
BoththeReporterandNegativeControlReporterareprovidedat55ngDNA/µl(500µleach)
Format:
assaykit
Instructionsforuse:
Seedatasheetfordetailedtransfectionandassayprotocols.
Storage/StABIlity:
Stableatleast12monthsfromdateofreceipt,whenstoredasdirected(-20°C)
Application(s):
•MonitorMAPK/ERKsignalingpathwayactivityandSRF-mediatedactivity.
•ScreenforactivatorsorinhibitorsoftheMAPK/ERKsignalingpathway.
•StudyeffectsofRNAiorgeneoverexpressionontheactivityoftheMAPK/ERKpathway.
•ScreenforactivatorsorinhibitorsoftheMAPK/ERKsignalingpathway.
•StudyeffectsofRNAiorgeneoverexpressionontheactivityoftheMAPK/ERKpathway.
Reference(s):
1.Wong,K.K.(2009)Recentdevelopmentsinanti-canceragentstargetingtheRas/Raf/MEK/ERKpathway.RecentPatAnticancerDrugDiscov.4(1):28-35.
2.Treisman,R.(1992)Theserumresponseelement.TrendsBiochemSci.17(10):423-426.
2.Treisman,R.(1992)Theserumresponseelement.TrendsBiochemSci.17(10):423-426.
Notes:
Thevectorsaresuitablefortransienttransfection.TheyareNOTsuitablefortransformationandamplificationinbacteria.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
CellSignalingPathway
ProductType:
ReporterAssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
