Bpsbioscience/NF-κB报告试剂盒（NF-κB信号通路）/60614/500反应
商品编号：
60614
品牌：
bpsbioscience
市场价：
¥11800.00
美元价：
7080.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
NF-κB(NuclearFactor-KappaB,NFkB)isaheterodimericproteincomposedofdifferentcombinationsofmembersoftheRelfamilyoftranscriptionfactors.TheNF-kB/Relfamilyoftranscriptionfactors(p50,p65,c-Rel,etc.)areinvolvedinstress,immune,andinflammatoryresponses.Inunstimulatedcells,theNF-κBdimersaresequesteredinthecytoplasmbyinhibitoryIkBproteins.Proinflammatorycytokines,LPS,growthfactors,andantigenreceptorsactivateIBkinase(IKK),whichphosphorylatestheIBproteins.PhosphorylationofIkBleadstoitsdegradation,freeingNF-κBcomplexestotranslocatetothenucleus,bindtoNF-κBDNAresponseelements,andinducethetranscriptionofthetargetgenes.
Description:
TheNF-κBReporterkitisdesignedformonitoringtheactivityoftheNF-κBsignalingpathwayintheculturedcells.Thekitcontainstransfection-readyNF-κBluciferasereportervector.ThisreportercontainsafireflyluciferasegeneunderthecontrolofmultimerizedNF-κBresponsiveelementlocatedupstreamofaminimalpromoter.TheNF-κBreporterispremixedwithconstitutively-expressingRenilla(seapansy)luciferasevector,whichservesasaninternalcontrolfortransfectionefficiency.Thekitalsoincludesanon-inducIBLefireflyluciferasevectorpremixedwithconstitutively-expressingRenillaluciferasevectorasanegativecontrol.Thenon-inducibleluciferasevectorcontainsafireflyluciferasegeneunderthecontrolofaminimalpromoter,withoutanyadditionalresponseelements.Thenegativecontroliscriticaltodeterminingpathwayspecificeffectsandbackgroundluciferaseactivity.
Synonym(s):
NF-kBreporter,NFkappaBreporter,luciferasereporter,NF-kBreporter,NFkBreporter
SuppliedAs:
Reporter:NF-κBluciferasereportervector+constitutively-expressingRenillaluciferasevector
NegativeControlReporter:Non-inducibleluciferasevector+constitutively-expressingRenillaluciferasevector
NegativeControlReporter:Non-inducibleluciferasevector+constitutively-expressingRenillaluciferasevector
Concentration:
BoththeReporterandNegativeControlReporterareprovidedat60ngDNA/µl(500µleach)
Format:
assaykit
Instructionsforuse:
Seedatasheetinsertfordetailedtransfectionandassayprotocols.
Storage/StABIlity:
Stableatleast12monthsfromdateofreceipt,whenstoredasdirected(-20°C)
Application(s):
•MonitorNF-κBsignalingpathwayactivity.
•ScreenforactivatorsorinhibitorsofNF-κBsignalingorNF-κBpathwaycomponents.
•StudyeffectsofRNAiorgeneoverexpressionontheactivityoftheNF-κBpathway.
•ScreenforactivatorsorinhibitorsofNF-κBsignalingorNF-κBpathwaycomponents.
•StudyeffectsofRNAiorgeneoverexpressionontheactivityoftheNF-κBpathway.
Reference(s):
1.Pessara,U.,Koch,N.(1990).MolCellBiol.10(8):4146-4154.
2.Baeuerle,P.A.(1998).CurrBiol.8(1):R19-R22.
ApplicationReference(s):
1. Isotrifoliolinhibitspro-inflammatorymediatorsbysuppressionofTLR/NF-κBandTLR/MAPKsignalinginLPS-inducedRAW264.7cells(2017)
2. TranscriptionfactorsSTAT6andKLF4implementmacrophagepolarizationviathedualcatalyticpowersofMCPIP(2015)
3.Effectsofangiotensin-(1-7)ontheproliferationandcollagensynthesisofargininevasopressin-stimulatedratcardiacfibroblasts:roleofmasreceptor-calcineurin-NF-κBsignalingpathway(2014)
Notes:
Thevectorsaresuitablefortransienttransfection.TheyareNOTsuitablefortransformationandamplificationinbacteria.
Warning(s):
Avoidfreeze/thawcycles
ProductType:
ReporterAssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
