Bpsbioscience/GAL4报告试剂盒（糖皮质激素受体途径）/60522/500反应-免疫在线蚂蚁淘旗下平台

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商品详细Bpsbioscience/GAL4报告试剂盒（糖皮质激素受体途径）/60522/500反应

Bpsbioscience/GAL4报告试剂盒（糖皮质激素受体途径）/60522/500反应

商品编号： 60522

品牌： bpsbioscience

市场价： ¥11300.00

美元价： 6780.00

产地：美国(厂家直采)

公司：

产品分类：其它检测试剂盒

公司分类： Other_kits

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电话号码： 4000-520-616

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商品介绍

Background:

Theglucocorticoidsignalingpathwayplaysanimportantroleindevelopment,fluidhomeostasis,cognition,immuneresponseandmetabolism.Glucocorticoidsareaclassofsteroidhormonesthatbindtotheglucocorticoidreceptor,causingittotranslocatetothenucleus.Upontranslocation,thereceptorcanregulatethetranscriptionofalargenumberofgenes,includingthosethatregulateglucosemetabolismandinflammatoryresponses.

Description:

TheGlucocorticoidReceptorPathwayReporterKitisdesignedformonitoringtheactivityoftheglucocorticoidsignalingpathwayinculturedcells.Thekitcontainsatransfection-readyexpressionvectorfortheglucocorticoidreceptorligandbindingdomainthatisfusedtotheDNAbindingdomain(DBD)ofGAL4(GAL4DBD-GR).ThisfusionconstructactivatesfireflyluciferaseexpressionunderthecontrolofamultimerizedGAL4upstreamactivationsequence(UAS).Thisallowsforspecificdetectionofglucocorticoid-inducedactivationoftheglucocorticoidreceptorwithouttheneedforindividualtranscriptionaltargetsandwithlowcrossreactivityforothernuclearreceptorpathways.TheGAL4/UASreporterispremixedwithconstitutivelyexpressingRenilla(seapansy)luciferasevector,whichservesasaninternalpositivecontrolfortransfectionefficiency.

Thekitalsoincludesanon-inducIBLefireflyluciferasevectorpremixedwithconstitutivelyexpressingRenillaluciferasevectorasanegativecontrol.Thenon-inducibleluciferasevectorcontainsafireflyluciferasegeneunderthecontrolofaminimalpromoter,butwithoutanyadditionalresponseelements.Thenegativecontroliscriticalfordeterminingpathway-specificeffectsandbackgroundluciferaseactivity.

Synonym(s):

GlucocorticoidReceptor,GR,Nuclearreceptorsubfamily3groupCmember1,NR3C1,GAL4,GAL4/UAS

Format:

COMPONENTS:

Instructionsforuse:

Seeassaykitdatasheetfordetailedprotocol.

Storage/StABIlity:

>1yearfromdateofreceiptwhenstoredat-20°C

Application(s):

•Monitorglucocorticoidsignalingpathwayactivity.•Screenactivatorsorinhibitorsoftheglucocorticoidsignalingpathway.

Reference(s):

PaguioA,etal.(2010)ImprovedDual-LuciferaseReporterAssaysforNuclearReceptors.CurrChemGenomics.4:43-49.

Notes:

MaterialsRequiredbutNotSupplied
•Mammaliancelllineandappropriatecellculturemedium
•96-welltissuecultureplateor96-welltissueculturetreatedwhiteclear-bottomassayplate(Corning#3610)
•Transfectionreagentformammaliancellline.[WeuseLipofectamine™2000(LifeTechnologies#11668027),however,othertransfectionreagentsshouldworkequallywell.]
•Opti-MEMIReducedSerumMedium(LifeTechnologies#31985-062)
•Dualluciferaseassaysystem:Dual-Glo®LuciferaseAssaySystem(Promega#E2920):Thissystemassayscellsdirectlyingrowthmedium.Itcanbeusedwithanyluminometer.Automatedinjectorsarenotrequired.ORDual-Luciferase®ReporterAssaySystem(Promega#E1910):Thissystemrequiresacelllysisstep.Itisidealforluminometerswithautomatedinjectors.
•Luminometer

Thesevectorsarereadyfortransienttransfection.TheyareNOTSUITABLEfortransformationandamplificationinbacteria.

Warning(s):

Avoidfreeze/thawcycles.

ScientificCategory:

Cell-BasedAssayKit

ProductType:

AssayKit

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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