Bpsbioscience/CRE/CREB报告试剂盒(cAMP/PKA信号通路)/60611/500反应
商品编号:
60611
品牌:
bpsbioscience
市场价:
¥11800.00
美元价:
7080.00
产品分类:
其它检测试剂盒
公司分类:
Other_kits
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Description:
TheCRE/CREBReporterkitisdesignedformonitoringtheactivityofthecAMP/PKAsignalingpathwayinculturedcells.Thekitcontainstransfection-readyCREluciferasereporter.ThisreportercontainsthefireflyluciferasegeneunderthecontrolofmultimerizedcAMPresponseelement(CRE)locatedupstreamofaminimalpromoter.ElevationoftheintracellularcAMPlevelactivatescAMPresponseelementbindingprotein(CREB)tobindCREandinducestheexpressionofluciferase.TheCREreporterispremixedwithconstitutively-expressingRenilla(seapansy)luciferasevectorthatservesasaninternalcontrolfortransfectionefficiency.Thekitalsoincludesanon-inducIBLefireflyluciferasevectorpremixedwiththeconstitutivelyexpressingRenillaluciferasevectorasanegativecontrol.Thenon-inducibleluciferasevectorcontainsthefireflyluciferasegeneunderthecontrolofaminimalpromoter,butwithoutanyadditionalresponseelements.Thenegativecontroliscriticaltodeterminingpathway-specificeffectsandbackgroundluciferaseactivity.
Synonym(s):
CREreporter,CREBreporter,cAMPreporter,PKAreporter,cAMPresponseelement
SuppliedAs:
Reporter:CREluciferasereportervector+constitutively-expressingRenillaluciferasevector
NegativeControlReporter:Non-inducibleluciferasevector+constitutively-expressingRenillaluciferasevector
NegativeControlReporter:Non-inducibleluciferasevector+constitutively-expressingRenillaluciferasevector
Concentration:
BoththeReporterandNegativeControlReporterareprovidedat60ngDNA/µl(500µleach)
Format:
assaykit
Instructionsforuse:
Seedatasheetfordetailedtransfectionandassayprotocols.
Storage/StABIlity:
Stableatleast12monthsfromdateofreceipt,whenstoredasdirected(-20°C)
Application(s):
•MonitortheJNKsignalingpathwayactivityandAP1-mediatedactivity.
•ScreenforactivatorsorinhibitorsoftheJNKsignalingpathway.
•StudyeffectsofRNAiorgeneoverexpressionontheactivityoftheJNKpathway.
•ScreenforactivatorsorinhibitorsoftheJNKsignalingpathway.
•StudyeffectsofRNAiorgeneoverexpressionontheactivityoftheJNKpathway.
Reference(s):
1.Montminy,M.R.etal.(1987)Bindingofanuclearproteintothecyclic-AMPresponseelementofthesomatostatingene.Nature328(6126):175-178
2.FanChung,K.(2006)Phosphodiesteraseinhibitorsinairwaysdisease.Eur.J.Pharmacol.533(1-3):110-117
3.Malik,R.etal.(2008)Cloning,stableexpressionofhumanphosphodiesterase7AanddevelopmentofanassayforscreeningofPDE7selectiveinhibitors.Appl.Microbiol.Biotechnol.77(5):1167-1173
2.FanChung,K.(2006)Phosphodiesteraseinhibitorsinairwaysdisease.Eur.J.Pharmacol.533(1-3):110-117
3.Malik,R.etal.(2008)Cloning,stableexpressionofhumanphosphodiesterase7AanddevelopmentofanassayforscreeningofPDE7selectiveinhibitors.Appl.Microbiol.Biotechnol.77(5):1167-1173
ApplicationReferences:
1.NuclearTRAF3isanegativeregulatorofCREBinBcells(2016)
Notes:
Thevectorsaresuitablefortransienttransfection.TheyareNOTsuitablefortransformationandamplificationinbacteria.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
CellSignalingPathway
ProductType:
ReporterAssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。