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Bpsbioscience/TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)/80578/96 reactions
Bpsbioscience/TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)/80578/96 reactions
商品编号:
80578
品牌:
bpsbioscience
市场价:
¥15500.00
美元价:
9300.00
产品分类:
其它检测试剂盒
公司分类:
Other_kits
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Background:
TNKS2catalyzestheNAD-dependentadditionofpoly(ADP-ribose)tothesubstrateproteins.
Description:
TheTNKS2HistoneRibosylationAssayKit(Biotin-labeledNAD+)isdesignedtomeasureTankyrase2(TNKS2)activityforscreeningandprofilingapplications.ThekeytotheTNKS2HistoneRibosylationAssayisthebiotinylatedNAD+substrate.Withthiskit,onlythreesimplestepsarerequiredforTNKS2reactions.First,histoneproteinsarecoatedona96-wellplate.Next,thebiotinylatedNAD+substrateisincubatedwithanassaybufferthatcontainstheTNKS2enzyme.Finally,theplateistreatedwithstreptavidin-HRPfollowedbyadditionoftheHRPsubstratetoproducechemiluminescencethatcanbemeasuredusingachemiluminescencereader.
Synonym(s):
Tankyrase2,TNKS2,poly(ADP-ribose)polymerase5,poly(ADP-ribosyl)transferase,PARP5b,PARP5c
SuppliedAs:
TheTNKS2assaykitcomesinaconvenient96-wellformat,withpurifiedTNKS2enzyme,histonemixture,andPARPassaybufferfor100enzymereactions.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:
COMPONENTS:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
Brown,J.A.,Marala,R.B.J.Pharmacol.Toxicol.Methods200247:137-41.
ApplicationReference(s):
DisruptionofWnt/β-CateninSignalingandTelomericShorteningAreInextricableConsequencesofTankyraseInhibitioninHumanCells(2015)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PARP
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。