Bpsbioscience/Anti–EzH2 polyclonal antibody/25223/50 µg-免疫在线蚂蚁淘旗下平台

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商品详细Bpsbioscience/Anti–EzH2 polyclonal antibody/25223/50 µg

Bpsbioscience/Anti–EzH2 polyclonal antibody/25223/50 µg

商品编号： 25223

品牌： bpsbioscience

市场价： ¥7500.00

美元价： 4500.00

产地：美国(厂家直采)

公司：

产品分类：多克隆抗体

公司分类： Polyclonal_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

HostSpecies:Rabbit

Clonality:polyclonal

Background:

EZH2(UniProt/Swiss–ProtentryQ15910)isahistone–lysinemethyltransferasewhichmethylatesLys–9andLys–27ofhistoneH3,leADIngtotranscriptionalrepression.Itisamemberofthepolycombgroup(PcG)familywhichformmultimericproteincomplexesandareinvolvedinmaintainingthetranscriptionalrepressivestateofgenesoversuccessivecellgenerations.TheEZH2activityisdependentontheassociationwithothercomponentsofthePRC2complex(EED,SUZ12/JJAZ1,RBBP4andRBBP7).EZH2mayplayaroleinthehematopoieticandcentralnervoussystems.Over–expressionofEZH2isobservedduringadvancedstagesofprostatecancerandbreastcancer.

Description:

PolyclonalantibodyraisedinrabbitagainsttheN–terminus(aa1–343)ofthemouseEZH2protein(Enhancerofzestehomolog2).

Synonym(s):

ENX–1,KMT6

AssayConditions:

ChIPresultsobtainedwiththeantibodydirectedagainstEZH2
ChIPassayswereperformedusingHeLacells,theantibodyagainstEZH2(Cat.#25223)andoptimizedPCRprimersetsforqPCR.ChIPwasperformedusingshearedchromatinfrom1millioncells.Atitrationoftheantibodyconsistingof0.5,1,2.5and5µgperChIPexperimentwasanalyzed.IgG(2µg/IP)wasusedasnegativeIPcontrol.QuantitativePCRwasperformedwithprimersforMYT1,usedasapositivecontroltarget,andforthepromoteroftheactiveGAPDHgene,usedasanegativecontrol.Figure1showstherecovery,expressedasa%ofinput(therelativeamountofimmunoprecipitatedDNAcomparedtoinputDNAafterqPCRanalysis).

WesternblotanalysisusingtheantibodydirectedagainstEZH2
NuclearextractsofHeLacells(40µg)wereanalyzedbyWesternblotusingtheantibodyagainstEZH2(Cat.#25223)diluted1:1,000inTBS-Tweencontaining5%skimmedmilk.Thepositionoftheproteinofinterest(expectedsize85kDa)isindicatedontheright;theMarker(inkDa)isshownontheleft.

Concentration:

50µg/50µl

Formulation:

PBScontaining0.05%azideand0.05%ProClin300

SpeciesReactivity:

Human,mouse

Purification:

ProteinGpurified

Immunogen:

recombinantprotein

Format:

Aqueousbuffersolution

Storage/StABIlity:

Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.

Application(s):

ChIP(2.5µg/ChIP)
WB(1:1000)
Immunochemistry(1:100–1:500)

Notes:

TheoptimalantibodyamountperIPshouldbedeterminedbytheend–user.Werecommendtesting1–5µgperIP

Warning(s):

Avoidfreeze/thawcycles

ScientificCategory:

Antibodies

ProductType:

Antibody

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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